NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/G|[alpha]| Export

@article{citeulike:4129719, abstract = {The spindle apparatus dictates the plane of cell cleavage, which is critical in the choice between symmetric or asymmetric division. Spindle positioning is controlled by an evolutionarily conserved pathway, which involves LIN-5/GPR-1/2/G in Caenorhabditis elegans, Mud/Pins/G in Drosophila and NuMA/LGN/G in humans1. GPR-1/2 and G localize LIN-5 to the cell cortex, which engages dynein and controls the cleavage plane during early mitotic divisions in C. elegans2, 3, 4, 5, 6. Here we identify ASPM-1 (abnormal spindle-like, microcephaly-associated) as a novel LIN-5 binding partner. ASPM-1, together with calmodulin (CMD-1), promotes meiotic spindle organization and the accumulation of LIN-5 at meiotic and mitotic spindle poles. Spindle rotation during maternal meiosis is independent of GPR-1/2 and G, yet requires LIN-5, ASPM-1, CMD-1 and dynein. Our data support the existence of two distinct LIN-5 complexes that determine localized dynein function: LIN-5/GPR-1/2/G at the cortex, and LIN-5/ASPM-1/CMD-1 at spindle poles. These functional interactions may be conserved in mammals, with implications for primary microcephaly.}, author = {van der Voet, Monique and Berends, Christian W. H. and Perreault, Audrey and Nguyen-Ngoc, Tu and Gonczy, Pierre and Vidal, Marc and Boxem, Mike and van den Heuvel, Sander}, citeulike-article-id = {4129719}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ncb1834}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ncb1834}, day = {15}, doi = {10.1038/ncb1834}, issn = {1465-7392}, journal = {Nature Cell Biology}, keywords = {meiosis, numa, spindle}, month = {February}, number = {3}, pages = {269--277}, posted-at = {2009-09-29 18:40:25}, priority = {2}, publisher = {Nature Publishing Group}, title = {NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/G|[alpha]|}, url = {http://dx.doi.org/10.1038/ncb1834}, volume = {11}, year = {2009} }

TY - JOUR ID - citeulike:4129719 L3 - citeulike-article-id:4129719 N2 - The spindle apparatus dictates the plane of cell cleavage, which is critical in the choice between symmetric or asymmetric division. Spindle positioning is controlled by an evolutionarily conserved pathway, which involves LIN-5/GPR-1/2/G in Caenorhabditis elegans, Mud/Pins/G in Drosophila and NuMA/LGN/G in humans1. GPR-1/2 and G localize LIN-5 to the cell cortex, which engages dynein and controls the cleavage plane during early mitotic divisions in C. elegans2, 3, 4, 5, 6. Here we identify ASPM-1 (abnormal spindle-like, microcephaly-associated) as a novel LIN-5 binding partner. ASPM-1, together with calmodulin (CMD-1), promotes meiotic spindle organization and the accumulation of LIN-5 at meiotic and mitotic spindle poles. Spindle rotation during maternal meiosis is independent of GPR-1/2 and G, yet requires LIN-5, ASPM-1, CMD-1 and dynein. Our data support the existence of two distinct LIN-5 complexes that determine localized dynein function: LIN-5/GPR-1/2/G at the cortex, and LIN-5/ASPM-1/CMD-1 at spindle poles. These functional interactions may be conserved in mammals, with implications for primary microcephaly. IS - 3 JF - Nature Cell Biology SN - 1465-7392 EP - 277 TI - NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/G|[alpha]| PB - Nature Publishing Group VL - 11 SP - 269 KW - meiosis KW - numa KW - spindle AU - van der Voet, Monique AU - Berends, Christian AU - Perreault, Audrey AU - Nguyen-Ngoc, Tu AU - Gonczy, Pierre AU - Vidal, Marc AU - Boxem, Mike AU - van den Heuvel, Sander PY - 2009/02/15/ UR - http://dx.doi.org/10.1038/ncb1834 ER -