Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Export

@article{citeulike:756, abstract = {The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.}, address = {Applied Biosystems, Foster City, California 94404, USA.}, author = {Livak, K. J. and Schmittgen, T. D.}, citeulike-article-id = {756}, citeulike-linkout-0 = {http://dx.doi.org/10.1006/meth.2001.1262}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1046-2023(01)91262-9}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11846609}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11846609}, doi = {10.1006/meth.2001.1262}, issn = {1046-2023}, journal = {Methods (San Diego, Calif.)}, keywords = {ct, del, method, pcr, real-time}, month = {December}, number = {4}, pages = {402--408}, posted-at = {2009-11-09 23:34:24}, priority = {2}, title = {Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.}, url = {http://dx.doi.org/10.1006/meth.2001.1262}, volume = {25}, year = {2001} }

TY - JOUR ID - citeulike:756 L3 - citeulike-article-id:756 N2 - The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data. IS - 4 JF - Methods (San Diego, Calif.) SN - 1046-2023 AD - Applied Biosystems, Foster City, California 94404, USA. EP - 408 TI - Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. VL - 25 SP - 402 KW - ct KW - del KW - method KW - pcr KW - real-time AU - Livak, KJ AU - Schmittgen, TD PY - 2001/12// UR - http://dx.doi.org/10.1006/meth.2001.1262 ER -