Environmental Genome Shotgun Sequencing of the Sargasso Sea Export

@article{citeulike:131325, abstract = {We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity. 10.1126/science.1093857}, author = {Venter, J. Craig and Remington, Karin and Heidelberg, John F. and Halpern, Aaron L. and Rusch, Doug and Eisen, Jonathan A. and Wu, Dongying and Paulsen, Ian and Nelson, Karen E. and Nelson, William and Fouts, Derrick E. and Levy, Samuel and Knap, Anthony H. and Lomas, Michael W. and Nealson, Ken and White, Owen and Peterson, Jeremy and Hoffman, Jeff and Parsons, Rachel and Baden-Tillson, Holly and Pfannkoch, Cynthia and Rogers, Yu-Hui and Smith, Hamilton O.}, citeulike-article-id = {131325}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1093857}, citeulike-linkout-1 = {http://www.sciencemag.org/content/sci;304/5667/66.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/sci;304/5667/66.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/304/5667/66}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/15001713}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=15001713}, citeulike-linkout-6 = {http://www.sciencemag.org/cgi/content/full/304/5667/66}, day = {2}, doi = {10.1126/science.1093857}, journal = {Science}, keywords = {ecology, molecular, sargasso, sequencing, shotgun}, month = {April}, number = {5667}, pages = {66--74}, posted-at = {2008-07-01 06:47:18}, priority = {2}, title = {Environmental Genome Shotgun Sequencing of the Sargasso Sea}, url = {http://dx.doi.org/10.1126/science.1093857}, volume = {304}, year = {2004} }

TY - JOUR ID - citeulike:131325 L3 - citeulike-article-id:131325 N2 - We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity. 10.1126/science.1093857 IS - 5667 JF - Science EP - 74 TI - Environmental Genome Shotgun Sequencing of the Sargasso Sea VL - 304 SP - 66 KW - ecology KW - molecular KW - sargasso KW - sequencing KW - shotgun AU - Venter, Craig AU - Remington, Karin AU - Heidelberg, John AU - Halpern, Aaron AU - Rusch, Doug AU - Eisen, Jonathan AU - Wu, Dongying AU - Paulsen, Ian AU - Nelson, Karen AU - Nelson, William AU - Fouts, Derrick AU - Levy, Samuel AU - Knap, Anthony AU - Lomas, Michael AU - Nealson, Ken AU - White, Owen AU - Peterson, Jeremy AU - Hoffman, Jeff AU - Parsons, Rachel AU - Baden-Tillson, Holly AU - Pfannkoch, Cynthia AU - Rogers, Yu-Hui AU - Smith, Hamilton PY - 2004/04/02/ UR - http://dx.doi.org/10.1126/science.1093857 ER -