Genotyping of Bacillus cereus Strains by Microarray-Based Resequencing Export

@article{citeulike:2960291, abstract = {The ability to distinguish microbial pathogens from closely related but nonpathogenic strains is key to understanding the population biology of these organisms. In this regard, Bacillus anthracis, the bacterium that causes inhalational anthrax, is of interest because it is closely related and often difficult to distinguish from other members of the B. cereus group that can cause diverse diseases. We employed custom-designed resequencing arrays (RAs) based on the genome sequence of Bacillus anthracis to generate 422 kb of genomic sequence from a panel of 41 Bacillus cereus sensu lato strains. Here we show that RAs represent a ” one reaction” genotyping technology with the ability to discriminate between highly similar B. anthracis isolates and more divergent strains of the B. cereus s.l. Clade 1. Our data show that RAs can be an efficient genotyping technology for pre-screening the genetic diversity of large strain collections to selected the best candidates for whole genome sequencing.}, author = {Zwick, Michael E. and Kiley, Maureen P. and Stewart, Andrew C. and Mateczun, Alfred and Read, Timothy D.}, citeulike-article-id = {2960291}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pone.0002513}, day = {2}, doi = {10.1371/journal.pone.0002513}, journal = {PLoS ONE}, keywords = {bacillus, genotyping, microarray}, month = {July}, number = {7}, pages = {e2513+}, posted-at = {2008-07-03 19:23:58}, priority = {2}, publisher = {Public Library of Science}, title = {Genotyping of Bacillus cereus Strains by Microarray-Based Resequencing}, url = {http://dx.doi.org/10.1371/journal.pone.0002513}, volume = {3}, year = {2008} }

TY - JOUR ID - citeulike:2960291 L3 - citeulike-article-id:2960291 N2 - The ability to distinguish microbial pathogens from closely related but nonpathogenic strains is key to understanding the population biology of these organisms. In this regard, Bacillus anthracis, the bacterium that causes inhalational anthrax, is of interest because it is closely related and often difficult to distinguish from other members of the B. cereus group that can cause diverse diseases. We employed custom-designed resequencing arrays (RAs) based on the genome sequence of Bacillus anthracis to generate 422 kb of genomic sequence from a panel of 41 Bacillus cereus sensu lato strains. Here we show that RAs represent a “one reaction” genotyping technology with the ability to discriminate between highly similar B. anthracis isolates and more divergent strains of the B. cereus s.l. Clade 1. Our data show that RAs can be an efficient genotyping technology for pre-screening the genetic diversity of large strain collections to selected the best candidates for whole genome sequencing. IS - 7 JF - PLoS ONE TI - Genotyping of Bacillus cereus Strains by Microarray-Based Resequencing PB - Public Library of Science VL - 3 SP - e2513 KW - bacillus KW - genotyping KW - microarray AU - Zwick, Michael AU - Kiley, Maureen AU - Stewart, Andrew AU - Mateczun, Alfred AU - Read, Timothy PY - 2008/07/02/ UR - http://dx.doi.org/10.1371/journal.pone.0002513 ER -