Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system Export

@article{citeulike:1851133, abstract = {Detailed analysis of neuronal network architecture requires the development of new methods. Here we present strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours. In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent proteins (XFPs). Integration of tandem Brainbow copies in transgenic mice yielded combinatorial XFP expression, and thus many colours, thereby providing a way to distinguish adjacent neurons and visualize other cellular interactions. As a demonstration, we reconstructed hundreds of neighbouring axons and multiple synaptic contacts in one small volume of a cerebellar lobe exhibiting approximately 90 colours. The expression in some lines also allowed us to map glial territories and follow glial cells and neurons over time in vivo. The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale.}, author = {Livet, Jean and Weissman, Tamily A. and Kang, Hyuno and Draft, Ryan W. and Lu, Ju and Bennis, Robyn A. and Sanes, Joshua R. and Lichtman, Jeff W.}, booktitle = {Nature}, citeulike-article-id = {1851133}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature06293}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature06293}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17972876}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17972876}, day = {01}, doi = {10.1038/nature06293}, issn = {0028-0836}, journal = {Nature}, keywords = {circuit\_busting, cre-lox, fluorescence, imaging, transgenic}, month = {November}, number = {7166}, pages = {56--62}, posted-at = {2007-11-03 12:16:04}, priority = {0}, publisher = {Nature Publishing Group}, title = {Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system}, url = {http://dx.doi.org/10.1038/nature06293}, volume = {450}, year = {2007} }

TY - JOUR ID - citeulike:1851133 L3 - citeulike-article-id:1851133 N2 - Detailed analysis of neuronal network architecture requires the development of new methods. Here we present strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours. In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent proteins (XFPs). Integration of tandem Brainbow copies in transgenic mice yielded combinatorial XFP expression, and thus many colours, thereby providing a way to distinguish adjacent neurons and visualize other cellular interactions. As a demonstration, we reconstructed hundreds of neighbouring axons and multiple synaptic contacts in one small volume of a cerebellar lobe exhibiting approximately 90 colours. The expression in some lines also allowed us to map glial territories and follow glial cells and neurons over time in vivo. The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale. IS - 7166 JF - Nature SN - 0028-0836 EP - 62 TI - Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system PB - Nature Publishing Group VL - 450 T2 - Nature SP - 56 KW - circuit_busting KW - cre-lox KW - fluorescence KW - imaging KW - transgenic AU - Livet, Jean AU - Weissman, Tamily AU - Kang, Hyuno AU - Draft, Ryan AU - Lu, Ju AU - Bennis, Robyn AU - Sanes, Joshua AU - Lichtman, Jeff PY - 2007/11/01/ UR - http://dx.doi.org/10.1038/nature06293 ER -