Small interfering RNA targeting the PINK1 induces apoptosis in dopaminergic cells SH-SY5Y Export

@article{citeulike:5925970, abstract = {PTEN-induced kinase 1 ( PINK1 ) is a recently identified gene, mutations of which cause levodopa-responsive parkinsonism. An over-expression of wild-type PINK1 protects neurons from stress-induced mitochondrial dysfunction and apoptosis. We studied the effects of PINK1 suppression using small interfering RNA (siRNA), which can inhibit PINK1 mRNA expression up to 87\%, and decrease PINK1 protein up to 80\% in human dopaminergic cell line SH-SY5Y. Incubation with PINK1 siRNA decreased SH-SY5Y cell viability and significantly increased MPP + or rotenone-induced cytotoxicity. Our results indicate that reduction in PINK1 expression can trigger apoptotic process that can be exacerbated by the presence of MPP + or rotenone. These findings support the hypothesis that PINK1 participates in the protection of dopaminergic neurons.}, author = {Deng, H. and Jankovic, J. and Guo, Y. and Xie, W. and Le, W.}, citeulike-article-id = {5925970}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.bbrc.2005.09.178}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0006291X05022205}, day = {02}, doi = {10.1016/j.bbrc.2005.09.178}, issn = {0006291X}, journal = {Biochemical and Biophysical Research Communications}, keywords = {dopamine, parkinsons, pink1, sirna}, month = {December}, number = {4}, pages = {1133--1138}, posted-at = {2009-10-12 11:16:27}, priority = {2}, title = {Small interfering RNA targeting the PINK1 induces apoptosis in dopaminergic cells SH-SY5Y}, url = {http://dx.doi.org/10.1016/j.bbrc.2005.09.178}, volume = {337}, year = {2005} }

TY - JOUR ID - citeulike:5925970 L3 - citeulike-article-id:5925970 N2 - PTEN-induced kinase 1 ( PINK1 ) is a recently identified gene, mutations of which cause levodopa-responsive parkinsonism. An over-expression of wild-type PINK1 protects neurons from stress-induced mitochondrial dysfunction and apoptosis. We studied the effects of PINK1 suppression using small interfering RNA (siRNA), which can inhibit PINK1 mRNA expression up to 87%, and decrease PINK1 protein up to 80% in human dopaminergic cell line SH-SY5Y. Incubation with PINK1 siRNA decreased SH-SY5Y cell viability and significantly increased MPP + or rotenone-induced cytotoxicity. Our results indicate that reduction in PINK1 expression can trigger apoptotic process that can be exacerbated by the presence of MPP + or rotenone. These findings support the hypothesis that PINK1 participates in the protection of dopaminergic neurons. IS - 4 JF - Biochemical and Biophysical Research Communications SN - 0006291X EP - 1138 TI - Small interfering RNA targeting the PINK1 induces apoptosis in dopaminergic cells SH-SY5Y VL - 337 SP - 1133 KW - dopamine KW - parkinsons KW - pink1 KW - sirna AU - Deng, H AU - Jankovic, J AU - Guo, Y AU - Xie, W AU - Le, W PY - 2005/12/02/ UR - http://dx.doi.org/10.1016/j.bbrc.2005.09.178 ER -