Genome scan for human obesity and linkage to markers in 20q13. Export

@article{lee99, abstract = {Obesity is a highly prevalent, multigenic trait that predicts increased morbidity and mortality. Here we report results from a genome scan based on 354 markers in 513 members of 92 nuclear families ascertained through extreme obesity and normal body weight. The average marker interval was approximately 10 cM. We examined four correlated obesity phenotypes, including the body-mass index (BMI) (both as a quantitative trait and as a discrete trait with a threshold of BMI > or /=30 kg/m2) and percentage of fat (both as a quantitative trait and as a discrete trait with a threshold of 40\%) as assessed by bioelectrical impedance. In the initial stage of the genome scan, four markers in 20q gave positive evidence for linkage, which was consistent across most obesity phenotypes and analytic methods. After saturating 20q with additional markers (25 markers total) in an augmented sample of 713 members from 124 families, we found linkage to several markers in a region, 20q13, previously implicated in both human and animal studies. Three markers (D20S107, D20S211, and D20S149) in 20q13 had empirical P values (based on Monte Carlo simulations, which controlled for multiple testing) < or /=. 01 for single-point analysis. In addition, the parametric, affecteds-only analysis for D20S476 yielded a LOD score of 3.06 (P=. 00009), and the affected-sib-pair test yielded a LOD score of 3.17 (P=.000067). Multipoint analyses further strengthened and localized these findings. This region includes several plausible candidate genes for obesity. Our results suggest that one or more genes affecting obesity are located in 20q13.}, author = {Lee, J. H. and Reed, D. R. and Li, W. D. and Xu, W. and Joo, E. J. and Kilker, R. L. and Nanthakumar, E. and North, M. and Sakul, H. and Bell, C. and Price, R. A.}, citeulike-article-id = {1437355}, journal = {Am J Hum Genet}, keywords = {african, ancestry, banding, chromosome, chromosomes, combinedbib, continental, european, frequency, gene, genetic, genetics, genotype, govt, group, heritable, human, humans, linkage, lod, mapping, markers, non-us, obesity, pair, phenotype, phs, quantitative, research, score, software, support, trait, us}, month = {Jan}, number = {1}, pages = {196--209}, pdf = {papers/ibdscore/obesity/lee99.pdf}, posted-at = {2007-07-05 14:55:15}, priority = {2}, title = {Genome scan for human obesity and linkage to markers in 20q13.}, volume = {64}, year = {1999} }

TY - JOUR ID - lee99 L3 - citeulike-article-id:1437355 N2 - Obesity is a highly prevalent, multigenic trait that predicts increased morbidity and mortality. Here we report results from a genome scan based on 354 markers in 513 members of 92 nuclear families ascertained through extreme obesity and normal body weight. The average marker interval was approximately 10 cM. We examined four correlated obesity phenotypes, including the body-mass index (BMI) (both as a quantitative trait and as a discrete trait with a threshold of BMI > or /=30 kg/m2) and percentage of fat (both as a quantitative trait and as a discrete trait with a threshold of 40\%) as assessed by bioelectrical impedance. In the initial stage of the genome scan, four markers in 20q gave positive evidence for linkage, which was consistent across most obesity phenotypes and analytic methods. After saturating 20q with additional markers (25 markers total) in an augmented sample of 713 members from 124 families, we found linkage to several markers in a region, 20q13, previously implicated in both human and animal studies. Three markers (D20S107, D20S211, and D20S149) in 20q13 had empirical P values (based on Monte Carlo simulations, which controlled for multiple testing) < or /=. 01 for single-point analysis. In addition, the parametric, affecteds-only analysis for D20S476 yielded a LOD score of 3.06 (P=. 00009), and the affected-sib-pair test yielded a LOD score of 3.17 (P=.000067). Multipoint analyses further strengthened and localized these findings. This region includes several plausible candidate genes for obesity. Our results suggest that one or more genes affecting obesity are located in 20q13. IS - 1 JF - Am J Hum Genet EP - 209 TI - Genome scan for human obesity and linkage to markers in 20q13. VL - 64 SP - 196 KW - african KW - ancestry KW - banding KW - chromosome KW - chromosomes KW - combinedbib KW - continental KW - european KW - frequency KW - gene KW - genetic KW - genetics KW - genotype KW - govt KW - group KW - heritable KW - human KW - humans KW - linkage KW - lod KW - mapping KW - markers KW - non-us KW - obesity KW - pair KW - phenotype KW - phs KW - quantitative KW - research KW - score KW - software KW - support KW - trait KW - us AU - Lee, JH AU - Reed, DR AU - Li, WD AU - Xu, W AU - Joo, EJ AU - Kilker, RL AU - Nanthakumar, E AU - North, M AU - Sakul, H AU - Bell, C AU - Price, RA PY - 1999/Jan// ER -