Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement? Export

@article{citeulike:305184, abstract = {We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be approximately 8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.}, address = {Biophysics Center, University of Illinois, Urbana, IL 61801, USA.}, author = {Kural, C. and Kim, H. and Syed, S. and Goshima, G. and Gelfand, V. I. and Selvin, P. R.}, citeulike-article-id = {305184}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1108408}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/308/5727/1469?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&searchid=1120767883833_8699&stored_search=&FIRSTINDEX=0&volume=308&firstpage=1469&fdate=10}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15817813}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15817813}, comment = {e27}, day = {3}, doi = {10.1126/science.1108408}, issn = {1095-9203}, journal = {Science}, keywords = {coordination, dynein, experiment, kinesin, movement}, month = {June}, number = {5727}, pages = {1469--1472}, posted-at = {2007-07-25 04:17:19}, priority = {0}, title = {Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement?}, url = {http://dx.doi.org/10.1126/science.1108408}, volume = {308}, year = {2005} }

TY - JOUR ID - citeulike:305184 L3 - citeulike-article-id:305184 N2 - We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be approximately 8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed. IS - 5727 JF - Science SN - 1095-9203 AD - Biophysics Center, University of Illinois, Urbana, IL 61801, USA. EP - 1472 TI - Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement? VL - 308 SP - 1469 KW - coordination KW - dynein KW - experiment KW - kinesin KW - movement N1 - e27 AU - Kural, C AU - Kim, H AU - Syed, S AU - Goshima, G AU - Gelfand, VI AU - Selvin, PR PY - 2005/06/03/ UR - http://dx.doi.org/10.1126/science.1108408 ER -