Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast. Export

@article{citeulike:1375672, abstract = {Microtubule (MT) nucleation not only occurs from centrosomes, but also in large part from dispersed nucleation sites. The subsequent sorting of short MTs into networks like the mitotic spindle requires molecular motors that laterally slide overlapping MTs and bundling proteins that statically connect MTs. How bundling proteins interfere with MT sliding is unclear. In bipolar MT bundles in fission yeast, we found that the bundler ase1p localized all along the length of antiparallel MTs, whereas the motor klp2p (kinesin-14) accumulated only at MT plus ends. Consequently, sliding forces could only overcome resistant bundling forces for short, newly nucleated MTs, which were transported to their correct position within bundles. Ase1p thus regulated sliding forces based on polarity and overlap length, and computer simulations showed these mechanisms to be sufficient to generate stable bipolar bundles. By combining motor and bundling proteins, cells can thus dynamically organize stable regions of overlap between cytoskeletal filaments.}, address = {Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.}, author = {Janson, M. E. and Loughlin, R. and Lo\"{i}odice, I. and Fu, C. and Brunner, D. and N\'{e}d\'{e}lec, F. J. and Tran, P. T.}, citeulike-article-id = {1375672}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2006.12.030}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17254972}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17254972}, citeulike-linkout-3 = {http://www.sciencedirect.com/science/article/B6WSN-4MWYF5C-M/2/102d2232e24bcc783830ae621e9e5021}, day = {26}, doi = {10.1016/j.cell.2006.12.030}, issn = {0092-8674}, journal = {Cell}, keywords = {array, bipolar, crosslinkers, dynamics, microtubule, motors, pombe}, month = {January}, number = {2}, pages = {357--368}, posted-at = {2007-07-02 10:53:07}, priority = {5}, title = {Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast.}, url = {http://dx.doi.org/10.1016/j.cell.2006.12.030}, volume = {128}, year = {2007} }

TY - JOUR ID - citeulike:1375672 L3 - citeulike-article-id:1375672 N2 - Microtubule (MT) nucleation not only occurs from centrosomes, but also in large part from dispersed nucleation sites. The subsequent sorting of short MTs into networks like the mitotic spindle requires molecular motors that laterally slide overlapping MTs and bundling proteins that statically connect MTs. How bundling proteins interfere with MT sliding is unclear. In bipolar MT bundles in fission yeast, we found that the bundler ase1p localized all along the length of antiparallel MTs, whereas the motor klp2p (kinesin-14) accumulated only at MT plus ends. Consequently, sliding forces could only overcome resistant bundling forces for short, newly nucleated MTs, which were transported to their correct position within bundles. Ase1p thus regulated sliding forces based on polarity and overlap length, and computer simulations showed these mechanisms to be sufficient to generate stable bipolar bundles. By combining motor and bundling proteins, cells can thus dynamically organize stable regions of overlap between cytoskeletal filaments. IS - 2 JF - Cell SN - 0092-8674 AD - Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA. EP - 368 TI - Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast. VL - 128 SP - 357 KW - array KW - bipolar KW - crosslinkers KW - dynamics KW - microtubule KW - motors KW - pombe AU - Janson, ME AU - Loughlin, R AU - Loïodice, I AU - Fu, C AU - Brunner, D AU - Nédélec, FJ AU - Tran, PT PY - 2007/01/26/ UR - http://dx.doi.org/10.1016/j.cell.2006.12.030 ER -