A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension. Export

@article{citeulike:1428319, abstract = {Proper connections between centromeres and spindle microtubules are of critical importance in ensuring accurate segregation of the genome during cell division. Using an in vitro approach based on the sequence-specific budding yeast centromere, we identified a complex of the chromosomal passenger proteins Bir1 and Sli15 (Survivin and INCENP) that links centromeres to microtubules. This linkage does not require Ipl1/Aurora B kinase, whose targeting and activation are controlled by Bir1 and Sli15. Ipl1 is the tension-dependent regulator of centromere-microtubule interactions that ensures chromosome biorientation on the spindle. Elimination of the linkage between centromeres and microtubules mediated by Bir1-Sli15 phenocopies mutations that selectively cripple Ipl1 kinase activation. These findings lead us to propose that the Bir1-Sli15-mediated linkage, which bridges centromeres and microtubules and includes the Aurora kinase-activating domain of INCENP family proteins, is the tension sensor that relays the mechanical state of centromere-microtubule attachments into local control of Ipl1 kinase activity.}, address = {Ludwig Institute for Cancer Research/Department of Cellular and Molecular Medicine, CMM-E Room 3052, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093, USA.}, author = {Sandall, S. and Severin, F. and McLeod, I. X. and Yates, J. R. and Oegema, K. and Hyman, A. and Desai, A.}, citeulike-article-id = {1428319}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2006.09.049}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17174893}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17174893}, day = {15}, doi = {10.1016/j.cell.2006.09.049}, issn = {0092-8674}, journal = {Cell}, keywords = {centromere, kinetochore, microtubule, spindle, tension}, month = {December}, number = {6}, pages = {1179--1191}, posted-at = {2007-07-02 11:02:37}, priority = {2}, title = {A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension.}, url = {http://dx.doi.org/10.1016/j.cell.2006.09.049}, volume = {127}, year = {2006} }

TY - JOUR ID - citeulike:1428319 L3 - citeulike-article-id:1428319 N2 - Proper connections between centromeres and spindle microtubules are of critical importance in ensuring accurate segregation of the genome during cell division. Using an in vitro approach based on the sequence-specific budding yeast centromere, we identified a complex of the chromosomal passenger proteins Bir1 and Sli15 (Survivin and INCENP) that links centromeres to microtubules. This linkage does not require Ipl1/Aurora B kinase, whose targeting and activation are controlled by Bir1 and Sli15. Ipl1 is the tension-dependent regulator of centromere-microtubule interactions that ensures chromosome biorientation on the spindle. Elimination of the linkage between centromeres and microtubules mediated by Bir1-Sli15 phenocopies mutations that selectively cripple Ipl1 kinase activation. These findings lead us to propose that the Bir1-Sli15-mediated linkage, which bridges centromeres and microtubules and includes the Aurora kinase-activating domain of INCENP family proteins, is the tension sensor that relays the mechanical state of centromere-microtubule attachments into local control of Ipl1 kinase activity. IS - 6 JF - Cell SN - 0092-8674 AD - Ludwig Institute for Cancer Research/Department of Cellular and Molecular Medicine, CMM-E Room 3052, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093, USA. EP - 1191 TI - A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension. VL - 127 SP - 1179 KW - centromere KW - kinetochore KW - microtubule KW - spindle KW - tension AU - Sandall, S AU - Severin, F AU - McLeod, IX AU - Yates, JR AU - Oegema, K AU - Hyman, A AU - Desai, A PY - 2006/12/15/ UR - http://dx.doi.org/10.1016/j.cell.2006.09.049 ER -