Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Export

@article{citeulike:1428325, abstract = {Mitotic cells face the challenging tasks of linking kinetochores to growing and shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome segregation. We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability. Microinjection of an antibody to the N terminus of Hec1 suppresses both microtubule detachment and microtubule plus-end polymerization and depolymerization at kinetochores of PtK1 cells. Centromeres become hyperstretched, kinetochore fibers shorten from spindle poles, kinetochore microtubule attachment errors increase, and chromosomes severely mis-segregate. The N terminus of Hec1 is phosphorylated by Aurora B kinase in vitro, and cells expressing N-terminal nonphosphorylatable mutants of Hec1 exhibit an increase in merotelic attachments, hyperstretching of centromeres, and errors in chromosome segregation. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules.}, address = {Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. jgdeluca@email.unc.edu}, author = {DeLuca, J. G. and Gall, W. E. and Ciferri, C. and Cimini, D. and Musacchio, A. and Salmon, E. D.}, citeulike-article-id = {1428325}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2006.09.047}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17129782}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17129782}, citeulike-linkout-3 = {http://www.sciencedirect.com/science/article/B6WSN-4MG0YV3-H/1/a117e357fae7e0a21ba4665439477c07}, day = {1}, doi = {10.1016/j.cell.2006.09.047}, issn = {0092-8674}, journal = {Cell}, keywords = {attachment, dynamics, kinetochore, microtubule, stability}, month = {December}, number = {5}, pages = {969--982}, posted-at = {2007-07-02 11:05:32}, priority = {2}, title = {Kinetochore microtubule dynamics and attachment stability are regulated by Hec1.}, url = {http://dx.doi.org/10.1016/j.cell.2006.09.047}, volume = {127}, year = {2006} }

TY - JOUR ID - citeulike:1428325 L3 - citeulike-article-id:1428325 N2 - Mitotic cells face the challenging tasks of linking kinetochores to growing and shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome segregation. We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability. Microinjection of an antibody to the N terminus of Hec1 suppresses both microtubule detachment and microtubule plus-end polymerization and depolymerization at kinetochores of PtK1 cells. Centromeres become hyperstretched, kinetochore fibers shorten from spindle poles, kinetochore microtubule attachment errors increase, and chromosomes severely mis-segregate. The N terminus of Hec1 is phosphorylated by Aurora B kinase in vitro, and cells expressing N-terminal nonphosphorylatable mutants of Hec1 exhibit an increase in merotelic attachments, hyperstretching of centromeres, and errors in chromosome segregation. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules. IS - 5 JF - Cell SN - 0092-8674 AD - Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. jgdeluca@email.unc.edu EP - 982 TI - Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. VL - 127 SP - 969 KW - attachment KW - dynamics KW - kinetochore KW - microtubule KW - stability AU - DeLuca, JG AU - Gall, WE AU - Ciferri, C AU - Cimini, D AU - Musacchio, A AU - Salmon, ED PY - 2006/12/01/ UR - http://dx.doi.org/10.1016/j.cell.2006.09.047 ER -