Molecular motor-induced instabilities and crosslinkers determine biopolymer organization. Export

@article{citeulike:1446273, abstract = {All eukaryotic cells rely on the active self-organization of protein filaments to form a responsive intracellular cytoskeleton. The necessity of motility and reaction to stimuli additionally requires pathways that quickly and reversibly change cytoskeletal organization. While thermally-driven order-disorder transitions are, from the viewpoint of physics, the most obvious method for controlling states of organization, the timescales necessary for effective cellular dynamics would require temperatures exceeding the physiologically viable temperature range. We report a mechanism whereby the molecular motor myosin II can cause near-instantaneous order-disorder transitions in reconstituted cytoskeletal actin solutions. When motor-induced filament sliding diminishes, the actin network structure rapidly and reversibly self-organizes into various assemblies. Addition of stable crosslinkers was found to alter the architectures of ordered assemblies. These isothermal transitions between dynamic disorder and self-assembled ordered states illustrate that the interplay between passive crosslinking and molecular motor activity plays a substantial role in dynamic cellular organization.}, address = {Institute for Soft Matter Physics, University of Leipzig.}, author = {Smith, David M M. and Ziebert, Falko and Humphrey, David and Duggan, Cynthia and Steinbeck, Matthias and Zimmermann, Walter and K\"{a}s, Josef A A.}, citeulike-article-id = {1446273}, citeulike-linkout-0 = {http://dx.doi.org/10.1529/biophysj.106.095919}, citeulike-linkout-1 = {http://www.biophysj.org/cgi/content/abstract/93/12/4445}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17604319}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17604319}, day = {29}, doi = {10.1529/biophysj.106.095919}, issn = {0006-3495}, journal = {Biophys J}, keywords = {biopolymer, disorder, organization, transitions}, month = {June}, posted-at = {2007-07-10 11:47:38}, priority = {3}, title = {Molecular motor-induced instabilities and crosslinkers determine biopolymer organization.}, url = {http://dx.doi.org/10.1529/biophysj.106.095919}, year = {2007} }

TY - JOUR ID - citeulike:1446273 L3 - citeulike-article-id:1446273 N2 - All eukaryotic cells rely on the active self-organization of protein filaments to form a responsive intracellular cytoskeleton. The necessity of motility and reaction to stimuli additionally requires pathways that quickly and reversibly change cytoskeletal organization. While thermally-driven order-disorder transitions are, from the viewpoint of physics, the most obvious method for controlling states of organization, the timescales necessary for effective cellular dynamics would require temperatures exceeding the physiologically viable temperature range. We report a mechanism whereby the molecular motor myosin II can cause near-instantaneous order-disorder transitions in reconstituted cytoskeletal actin solutions. When motor-induced filament sliding diminishes, the actin network structure rapidly and reversibly self-organizes into various assemblies. Addition of stable crosslinkers was found to alter the architectures of ordered assemblies. These isothermal transitions between dynamic disorder and self-assembled ordered states illustrate that the interplay between passive crosslinking and molecular motor activity plays a substantial role in dynamic cellular organization. JF - Biophys J SN - 0006-3495 AD - Institute for Soft Matter Physics, University of Leipzig. TI - Molecular motor-induced instabilities and crosslinkers determine biopolymer organization. KW - biopolymer KW - disorder KW - organization KW - transitions AU - Smith, David M AU - Ziebert, Falko AU - Humphrey, David AU - Duggan, Cynthia AU - Steinbeck, Matthias AU - Zimmermann, Walter AU - Käs, Josef A PY - 2007/06/29/ UR - http://dx.doi.org/10.1529/biophysj.106.095919 ER -