Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations

@article{citeulike:1260189, abstract = {Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations. 10.1126/science.1132067}, author = {Ishii, Nobuyoshi and Nakahigashi, Kenji and Baba, Tomoya and Robert, Martin and Soga, Tomoyoshi and Kanai, Akio and Hirasawa, Takashi and Naba, Miki and Hirai, Kenta and Hoque, Aminul and Ho, Pei Y. and Kakazu, Yuji and Sugawara, Kaori and Igarashi, Saori and Harada, Satoshi and Masuda, Takeshi and Sugiyama, Naoyuki and Togashi, Takashi and Hasegawa, Miki and Takai, Yuki and Yugi, Katsuyuki and Arakawa, Kazuharu and Iwata, Nayuta and Toya, Yoshihiro and Nakayama, Yoichi and Nishioka, Takaaki and Shimizu, Kazuyuki and Mori, Hirotada and Tomita, Masaru}, citeulike-article-id = {1260189}, doi = {10.1126/science.1132067}, journal = {Science}, keywords = {defect, flux, ht, metabolic\_profiling, mutant, network, systems}, month = {April}, number = {5824}, pages = {593--597}, posted-at = {2007-04-27 13:34:19}, priority = {4}, title = {Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations}, url = {http://dx.doi.org/10.1126/science.1132067}, volume = {316}, year = {2007} }

TY - JOUR ID - citeulike:1260189 L3 - citeulike-article-id:1260189 N2 - Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations. 10.1126/science.1132067 IS - 5824 JF - Science EP - 597 TI - Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations VL - 316 SP - 593 KW - defect KW - flux KW - ht KW - metabolic_profiling KW - mutant KW - network KW - systems AU - Ishii, Nobuyoshi AU - Nakahigashi, Kenji AU - Baba, Tomoya AU - Robert, Martin AU - Soga, Tomoyoshi AU - Kanai, Akio AU - Hirasawa, Takashi AU - Naba, Miki AU - Hirai, Kenta AU - Hoque, Aminul AU - Ho, Pei AU - Kakazu, Yuji AU - Sugawara, Kaori AU - Igarashi, Saori AU - Harada, Satoshi AU - Masuda, Takeshi AU - Sugiyama, Naoyuki AU - Togashi, Takashi AU - Hasegawa, Miki AU - Takai, Yuki AU - Yugi, Katsuyuki AU - Arakawa, Kazuharu AU - Iwata, Nayuta AU - Toya, Yoshihiro AU - Nakayama, Yoichi AU - Nishioka, Takaaki AU - Shimizu, Kazuyuki AU - Mori, Hirotada AU - Tomita, Masaru PY - 2007/04/27/ UR - http://dx.doi.org/10.1126/science.1132067 ER -