Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Export

@article{citeulike:186934, abstract = {We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA. Each of these probes will define a locus. Loci can be expanded or contracted to include more or less polymorphism by further application of recombinant DNA technology. Suitably polymorphic loci can be tested for linkage relationships in human pedigrees by established methods; and loci can be arranged into linkage groups to form a true genetic map of "DNA marker loci." Pedigrees in which inherited traits are known to be segregating can then be analyzed, making possible the mapping of the gene(s) responsible for the trait with respect to the DNA marker loci, without requiring direct access to a specified gene's DNA. For inherited diseases mapped in this way, linked DNA marker loci can be used predictively for genetic counseling.}, author = {Botstein, D. and White, R. L. and Skolnick, M. and Davis, R. W.}, citeulike-article-id = {186934}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/6247908}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=6247908}, issn = {0002-9297}, journal = {Am J Hum Genet}, keywords = {basis, disease, genetic, mendelian, rflp, statistics}, month = {May}, number = {3}, pages = {314--331}, posted-at = {2008-01-17 14:30:30}, priority = {3}, title = {Construction of a genetic linkage map in man using restriction fragment length polymorphisms.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/6247908}, volume = {32}, year = {1980} }

TY - JOUR ID - citeulike:186934 L3 - citeulike-article-id:186934 N2 - We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA. Each of these probes will define a locus. Loci can be expanded or contracted to include more or less polymorphism by further application of recombinant DNA technology. Suitably polymorphic loci can be tested for linkage relationships in human pedigrees by established methods; and loci can be arranged into linkage groups to form a true genetic map of "DNA marker loci." Pedigrees in which inherited traits are known to be segregating can then be analyzed, making possible the mapping of the gene(s) responsible for the trait with respect to the DNA marker loci, without requiring direct access to a specified gene's DNA. For inherited diseases mapped in this way, linked DNA marker loci can be used predictively for genetic counseling. IS - 3 JF - Am J Hum Genet SN - 0002-9297 EP - 331 TI - Construction of a genetic linkage map in man using restriction fragment length polymorphisms. VL - 32 SP - 314 KW - basis KW - disease KW - genetic KW - mendelian KW - rflp KW - statistics AU - Botstein, D AU - White, RL AU - Skolnick, M AU - Davis, RW PY - 1980/05// UR - http://view.ncbi.nlm.nih.gov/pubmed/6247908 ER -