Exploration of essential gene functions via titratable promoter alleles. Export

@article{citeulike:201505, abstract = {Nearly 20\% of yeast genes are required for viability, hindering genetic analysis with knockouts. We created promoter-shutoff strains for over two-thirds of all essential yeast genes and subjected them to morphological analysis, size profiling, drug sensitivity screening, and microarray expression profiling. We then used this compendium of data to ask which phenotypic features characterized different functional classes and used these to infer potential functions for uncharacterized genes. We identified genes involved in ribosome biogenesis (HAS1, URB1, and URB2), protein secretion (SEC39), mitochondrial import (MIM1), and tRNA charging (GSN1). In addition, apparent negative feedback transcriptional regulation of both ribosome biogenesis and the proteasome was observed. We furthermore show that these strains are compatible with automated genetic analysis. This study underscores the importance of analyzing mutant phenotypes and provides a resource to complement the yeast knockout collection.}, address = {Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, ON M5G 1L6, Canada.}, author = {Mnaimneh, S. and Davierwala, A. P. and Haynes, J. and Moffat, J. and Peng, W. T. and Zhang, W. and Yang, X. and Pootoolal, J. and Chua, G. and Lopez, A. and Trochesset, M. and Morse, D. and Krogan, N. J. and Hiley, S. L. and Li, Z. and Morris, Q. and Grigull, J. and Mitsakakis, N. and Roberts, C. J. and Greenblatt, J. F. and Boone, C. and Kaiser, C. A. and Andrews, B. J. and Hughes, T. R.}, citeulike-article-id = {201505}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2004.06.013}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15242642}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15242642}, day = {9}, doi = {10.1016/j.cell.2004.06.013}, issn = {0092-8674}, journal = {Cell}, keywords = {deletion, mutant, regulation, yeast}, month = {July}, number = {1}, pages = {31--44}, posted-at = {2008-12-17 02:49:48}, priority = {4}, title = {Exploration of essential gene functions via titratable promoter alleles.}, url = {http://dx.doi.org/10.1016/j.cell.2004.06.013}, volume = {118}, year = {2004} }

TY - JOUR ID - citeulike:201505 L3 - citeulike-article-id:201505 N2 - Nearly 20% of yeast genes are required for viability, hindering genetic analysis with knockouts. We created promoter-shutoff strains for over two-thirds of all essential yeast genes and subjected them to morphological analysis, size profiling, drug sensitivity screening, and microarray expression profiling. We then used this compendium of data to ask which phenotypic features characterized different functional classes and used these to infer potential functions for uncharacterized genes. We identified genes involved in ribosome biogenesis (HAS1, URB1, and URB2), protein secretion (SEC39), mitochondrial import (MIM1), and tRNA charging (GSN1). In addition, apparent negative feedback transcriptional regulation of both ribosome biogenesis and the proteasome was observed. We furthermore show that these strains are compatible with automated genetic analysis. This study underscores the importance of analyzing mutant phenotypes and provides a resource to complement the yeast knockout collection. IS - 1 JF - Cell SN - 0092-8674 AD - Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, ON M5G 1L6, Canada. EP - 44 TI - Exploration of essential gene functions via titratable promoter alleles. VL - 118 SP - 31 KW - deletion KW - mutant KW - regulation KW - yeast AU - Mnaimneh, S AU - Davierwala, AP AU - Haynes, J AU - Moffat, J AU - Peng, WT AU - Zhang, W AU - Yang, X AU - Pootoolal, J AU - Chua, G AU - Lopez, A AU - Trochesset, M AU - Morse, D AU - Krogan, NJ AU - Hiley, SL AU - Li, Z AU - Morris, Q AU - Grigull, J AU - Mitsakakis, N AU - Roberts, CJ AU - Greenblatt, JF AU - Boone, C AU - Kaiser, CA AU - Andrews, BJ AU - Hughes, TR PY - 2004/07/09/ UR - http://dx.doi.org/10.1016/j.cell.2004.06.013 ER -