Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes Export

@article{citeulike:3459996, abstract = {10.1073/pnas.0806261105 Synthetic lethal genetic interaction networks define genes that work together to control essential functions and have been studied extensively in using the synthetic genetic array (SGA) analysis technique (SGA). The extent to which synthetic lethal or other genetic interaction networks are conserved between species remains uncertain. To address this question, we compared literature-curated and experimentally derived genetic interaction networks for two distantly related yeasts, and . We find that 23\% of interactions in a novel, high-quality literature-curated network are conserved in the existing network. Next, we developed a method, called SGA analysis (SGA), enabling rapid, high-throughput isolation of genetic interactions in this species. Direct comparison by SGA and SGA of \^{a}ˆ¼220 genes involved in DNA replication, the DNA damage response, chromatin remodeling, intracellular transport, and other processes revealed that \^{a}ˆ¼29\% of genetic interactions are common to both species, with the remainder exhibiting unique, species-specific patterns of genetic connectivity. We define a conserved yeast network (CYN) composed of 106 genes and 144 interactions and suggest that this network may help understand the shared biology of diverse eukaryotic species.}, author = {Dixon, Scott J. and Fedyshyn, Yaroslav and Koh, Judice L. Y. and Prasad, T. S. Keshava and Chahwan, Charly and Chua, Gordon and Toufighi, Kiana and Baryshnikova, Anastasija and Hayles, Jacqueline and Hoe, Kwang-Lae and Kim, Dong-Uk and Park, Han-Oh and Myers, Chad L. and Pandey, Akhilesh and Durocher, Daniel and Andrews, Brenda J. and Boone, Charles}, citeulike-article-id = {3459996}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0806261105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/43/16653.full.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/43/16653.full.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18931302}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18931302}, day = {28}, doi = {10.1073/pnas.0806261105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences}, keywords = {evolution, fg2008, ht, interaction, network, sl, yeast}, month = {October}, number = {43}, pages = {16653--16658}, posted-at = {2008-12-16 13:59:00}, priority = {0}, title = {Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes}, url = {http://dx.doi.org/10.1073/pnas.0806261105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3459996 L3 - citeulike-article-id:3459996 N2 - 10.1073/pnas.0806261105 Synthetic lethal genetic interaction networks define genes that work together to control essential functions and have been studied extensively in using the synthetic genetic array (SGA) analysis technique (SGA). The extent to which synthetic lethal or other genetic interaction networks are conserved between species remains uncertain. To address this question, we compared literature-curated and experimentally derived genetic interaction networks for two distantly related yeasts, and . We find that 23% of interactions in a novel, high-quality literature-curated network are conserved in the existing network. Next, we developed a method, called SGA analysis (SGA), enabling rapid, high-throughput isolation of genetic interactions in this species. Direct comparison by SGA and SGA of ∼220 genes involved in DNA replication, the DNA damage response, chromatin remodeling, intracellular transport, and other processes revealed that ∼29% of genetic interactions are common to both species, with the remainder exhibiting unique, species-specific patterns of genetic connectivity. We define a conserved yeast network (CYN) composed of 106 genes and 144 interactions and suggest that this network may help understand the shared biology of diverse eukaryotic species. IS - 43 JF - Proceedings of the National Academy of Sciences SN - 1091-6490 EP - 16658 TI - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes VL - 105 SP - 16653 KW - evolution KW - fg2008 KW - ht KW - interaction KW - network KW - sl KW - yeast AU - Dixon, Scott AU - Fedyshyn, Yaroslav AU - Koh, Judice AU - Prasad, Keshava AU - Chahwan, Charly AU - Chua, Gordon AU - Toufighi, Kiana AU - Baryshnikova, Anastasija AU - Hayles, Jacqueline AU - Hoe, Kwang-Lae AU - Kim, Dong-Uk AU - Park, Han-Oh AU - Myers, Chad AU - Pandey, Akhilesh AU - Durocher, Daniel AU - Andrews, Brenda AU - Boone, Charles PY - 2008/10/28/ UR - http://dx.doi.org/10.1073/pnas.0806261105 ER -