Integral membrane proteins in the mitochondrial outer membrane of <i>Saccharomyces cerevisiae</i> Export

@article{citeulike:4288696, abstract = {Mitochondria evolved from a bacterial endosymbiont ancestor in which the integral outer membrane proteins would have been 03B2-barrel structured within the plane of the membrane. Initial proteomics on the outer membrane from yeast mitochondria suggest that while most of the protein components are integral in the membrane, most of these mitochondrial proteins behave as if they have 03B1-helical transmembrane domains, rather than 03B2-barrels. These proteins are usually predicted to have a single 03B1-helical transmembrane segment at either the N- or C-terminus, however, more complex topologies are also seen. We purified the novel outer membrane protein Om14 and show it is encoded in the gene YBR230c. Protein sequencing revealed an intron is spliced from the transcript, and both transcription from the YBR230c gene and steady-state level of the Om14 protein is dramatically less in cells grown on glucose than in cells grown on nonfermentable carbon sources. Hydropathy predictions together with data from limited protease digestion show three 03B1-helical transmembrane segments in Om14. The 03B1-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane.}, address = {Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia; Bio21 Molecular Science and Biotechnology Institute, Parkville, Australia; Biozentrum, University of Basel, Switzerland}, author = {Burri, Lena and Vascotto, Katherine and Gentle, Ian E. and Chan, Nickie C. and Beilharz, Traude and Stapleton, David I. and Ramage, Lynn and Lithgow, Trevor}, citeulike-article-id = {4288696}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1742-4658.2006.05171.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/118635115/ABSTRACT}, doi = {10.1111/j.1742-4658.2006.05171.x}, journal = {FEBS Journal}, keywords = {basis, localization, mitochondria, morphology, parts-list, projmicr, proteomics}, number = {7}, pages = {1507--1515}, posted-at = {2009-04-08 14:13:51}, priority = {2}, title = {Integral membrane proteins in the mitochondrial outer membrane of <i>Saccharomyces cerevisiae</i>}, url = {http://dx.doi.org/10.1111/j.1742-4658.2006.05171.x}, volume = {273}, year = {2006} }

TY - JOUR ID - citeulike:4288696 L3 - citeulike-article-id:4288696 N2 - Mitochondria evolved from a bacterial endosymbiont ancestor in which the integral outer membrane proteins would have been 03B2-barrel structured within the plane of the membrane. Initial proteomics on the outer membrane from yeast mitochondria suggest that while most of the protein components are integral in the membrane, most of these mitochondrial proteins behave as if they have 03B1-helical transmembrane domains, rather than 03B2-barrels. These proteins are usually predicted to have a single 03B1-helical transmembrane segment at either the N- or C-terminus, however, more complex topologies are also seen. We purified the novel outer membrane protein Om14 and show it is encoded in the gene YBR230c. Protein sequencing revealed an intron is spliced from the transcript, and both transcription from the YBR230c gene and steady-state level of the Om14 protein is dramatically less in cells grown on glucose than in cells grown on nonfermentable carbon sources. Hydropathy predictions together with data from limited protease digestion show three 03B1-helical transmembrane segments in Om14. The 03B1-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane. IS - 7 JF - FEBS Journal AD - Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Australia; Bio21 Molecular Science and Biotechnology Institute, Parkville, Australia; Biozentrum, University of Basel, Switzerland EP - 1515 TI - Integral membrane proteins in the mitochondrial outer membrane of <i>Saccharomyces cerevisiae</i> VL - 273 SP - 1507 KW - basis KW - localization KW - mitochondria KW - morphology KW - parts-list KW - projmicr KW - proteomics AU - Burri, Lena AU - Vascotto, Katherine AU - Gentle, Ian AU - Chan, Nickie AU - Beilharz, Traude AU - Stapleton, David AU - Ramage, Lynn AU - Lithgow, Trevor PY - 2006/// UR - http://dx.doi.org/10.1111/j.1742-4658.2006.05171.x ER -