raiyar's Perea:J [2 articles]

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Limitations to in vivo import of hydrophobic proteins into yeast mitochondria. The case of a cytoplasmically synthesized apocytochrome b.

Eur J Biochem, Vol. 228, No. 3. (15 March 1995), pp. 762-771.

by M. G. Claros, J. Perea, Y. Shu, F. A. Samatey, J. L. Popot, C. Jacq

posted to allotopic basis cob example mitochondria mtdna mtknockout projmt technique yeast by raiyar on 2008-01-25 16:25:40

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The apocytochrome b gene, exclusively encoded by the mitochondrial genome, was engineered so that it could be expressed in the yeast cytoplasm. Different combinations of the apocytochrome b transmembrane domains were produced in the form of hybrid proteins fused to both the N-terminal mitochondrial targeting sequence of the ATPase subunit 9 from Neurospora crassa and to a cytoplasmic version of the bI4 RNA maturase, localised on the N-terminal and C-terminal sides, respectively, of the hydrophobic stretches. The bI4 RNA maturase, which ...

Allotopic expression of yeast mitochondrial maturase to study mitochondrial import of hydrophobic proteins.

Methods Enzymol, Vol. 264 (1996), pp. 389-403.

by M. G. Claros, J. Perea, C. Jacq

posted to allotopic example localization mitochondria mtdna projmt technique by raiyar on 2007-11-17 16:03:54

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raiyar's Perea:J [2 articles]

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