Comparison of comparative genomic hybridization technologies across microarray platforms. Export

@article{citeulike:4958941, abstract = {In the 2007 Association of Biomolecular Resource Facilities Microarray Research Group project, we analyzed HL-60 DNA with five platforms: Agilent, Affymetrix 500K, Affymetrix U133 Plus 2.0, Illumina, and RPCI 19K BAC arrays. Copy number variation was analyzed using circular binary segmentation (CBS) analysis of log ratio scores from four independently assessed hybridizations of each platform. Data obtained from these platforms were assessed for reproducibility and the ability to detect formerly reported copy number variations in HL-60. In HL-60, all of the tested platforms detected genomic DNA amplification of the 8q24 locus, trisomy 18, and monosomy X; and deletions at loci 5q11.2\~{}q31, 9p21.3\~{}p22, 10p12\~{}p15, 14q22\~{}q31, and 17p12\~{}p13.3. In the HL-60 genome, at least two of the five platforms detected five novel losses and five novel gains. This report provides guidance in the selection of platforms based on this wide-ranging evaluation of available CGH platforms.}, author = {Hester, Susan D. and Reid, Laura and Nowak, Norma and Jones, Wendell D. and Parker, Joel S. and Knudtson, Kevin and Ward, William and Tiesman, Jay and Denslow, Nancy D.}, citeulike-article-id = {4958941}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/19503625}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=19503625}, issn = {1943-4731}, journal = {Journal of biomolecular techniques : JBT}, keywords = {cgh, comparison, platform, review, technology}, month = {April}, number = {2}, pages = {135--151}, posted-at = {2009-06-29 17:22:06}, priority = {2}, title = {Comparison of comparative genomic hybridization technologies across microarray platforms.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/19503625}, volume = {20}, year = {2009} }

TY - JOUR ID - citeulike:4958941 L3 - citeulike-article-id:4958941 N2 - In the 2007 Association of Biomolecular Resource Facilities Microarray Research Group project, we analyzed HL-60 DNA with five platforms: Agilent, Affymetrix 500K, Affymetrix U133 Plus 2.0, Illumina, and RPCI 19K BAC arrays. Copy number variation was analyzed using circular binary segmentation (CBS) analysis of log ratio scores from four independently assessed hybridizations of each platform. Data obtained from these platforms were assessed for reproducibility and the ability to detect formerly reported copy number variations in HL-60. In HL-60, all of the tested platforms detected genomic DNA amplification of the 8q24 locus, trisomy 18, and monosomy X; and deletions at loci 5q11.2~q31, 9p21.3~p22, 10p12~p15, 14q22~q31, and 17p12~p13.3. In the HL-60 genome, at least two of the five platforms detected five novel losses and five novel gains. This report provides guidance in the selection of platforms based on this wide-ranging evaluation of available CGH platforms. IS - 2 JF - Journal of biomolecular techniques : JBT SN - 1943-4731 EP - 151 TI - Comparison of comparative genomic hybridization technologies across microarray platforms. VL - 20 SP - 135 KW - cgh KW - comparison KW - platform KW - review KW - technology AU - Hester, Susan AU - Reid, Laura AU - Nowak, Norma AU - Jones, Wendell AU - Parker, Joel AU - Knudtson, Kevin AU - Ward, William AU - Tiesman, Jay AU - Denslow, Nancy PY - 2009/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/19503625 ER -