Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis Export

@article{Winzeler1999, abstract = {The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.}, author = {Winzeler, Elizabeth A. and Shoemaker, Daniel D. and Astromoff, Anna and Liang, Hong and Anderson, Keith and Andre, Bruno and Bangham, Rhonda and Benito, Rocio and Boeke, Jef D. and Bussey, Howard and Chu, Angela M. and Connelly, Carla and Davis, Karen and Dietrich, Fred and Dow, Sally W. and Bakkoury, Mohamed E. and Foury, Francoise and Friend, Stephen H. and Gentalen, Erik and Giaever, Guri and Hegemann, Johannes H. and Jones, Ted and Laub, Michael and Liao, Hong and Liebundguth, Nicole and Lockhart, David J. and Lucau-Danila, Anca and Lussier, Marc and M'rabet, Nasiha and Menard, Patrice and Mittmann, Michael and Pai, Chai and Rebischung, Corinne and Revuelta, Jose L. and Riles, Linda and Roberts, Christopher J. and Ross-Macdonald, Petra and Scherens, Bart and Snyder, Michael and Sookhai-Mahadeo, Sharon and Storms, Reginald K. and Veronneau, Steeve and Voet, Marleen and Volckaert, Guido and Ward, Teresa R. and Wysocki, Robert and Yen, Grace S. and Yu, Kexin and Zimmermann, Katja and Philippsen, Peter and Johnston, Mark and Davis, Ronald W.}, citeulike-article-id = {4372046}, citeulike-linkout-0 = {http://proquest.umi.com.libproxy.mit.edu/pqdweb?index=0&did=43944141&SrchMode=1&sid=3&Fmt=4&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1240319909&clientId=5482}, day = {6}, issn = {00368075}, journal = {Science}, keywords = {functional, yeast}, month = {August}, number = {5429}, pages = {901--907}, posted-at = {2009-04-21 14:22:45}, priority = {0}, title = {Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis}, url = {http://proquest.umi.com.libproxy.mit.edu/pqdweb?index=0&did=43944141&SrchMode=1&sid=3&Fmt=4&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1240319909&clientId=5482}, volume = {285}, year = {1999} }

TY - JOUR ID - Winzeler1999 L3 - citeulike-article-id:4372046 N2 - The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium. IS - 5429 JF - Science SN - 00368075 EP - 907 TI - Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis VL - 285 SP - 901 KW - functional KW - yeast AU - Winzeler, Elizabeth AU - Shoemaker, Daniel AU - Astromoff, Anna AU - Liang, Hong AU - Anderson, Keith AU - Andre, Bruno AU - Bangham, Rhonda AU - Benito, Rocio AU - Boeke, Jef AU - Bussey, Howard AU - Chu, Angela AU - Connelly, Carla AU - Davis, Karen AU - Dietrich, Fred AU - Dow, Sally AU - Bakkoury, Mohamed AU - Foury, Francoise AU - Friend, Stephen AU - Gentalen, Erik AU - Giaever, Guri AU - Hegemann, Johannes AU - Jones, Ted AU - Laub, Michael AU - Liao, Hong AU - Liebundguth, Nicole AU - Lockhart, David AU - Lucau-Danila, Anca AU - Lussier, Marc AU - M'rabet, Nasiha AU - Menard, Patrice AU - Mittmann, Michael AU - Pai, Chai AU - Rebischung, Corinne AU - Revuelta, Jose AU - Riles, Linda AU - Roberts, Christopher AU - Ross-Macdonald, Petra AU - Scherens, Bart AU - Snyder, Michael AU - Sookhai-Mahadeo, Sharon AU - Storms, Reginald AU - Veronneau, Steeve AU - Voet, Marleen AU - Volckaert, Guido AU - Ward, Teresa AU - Wysocki, Robert AU - Yen, Grace AU - Yu, Kexin AU - Zimmermann, Katja AU - Philippsen, Peter AU - Johnston, Mark AU - Davis, Ronald PY - 1999/08/06/ UR - http://proquest.umi.com.libproxy.mit.edu/pqdweb?index=0&did=43944141&SrchMode=1&sid=3&Fmt=4&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1240319909&clientId=5482 ER -