A unique and universal molecular barcode array Export

by: Sarah E. Pierce, Eula L. Fung, Daniel F. Jaramillo, Angela M. Chu, Ronald W. Davis, Corey Nislow, Guri Giaever

Nature Methods, Vol. 3, No. 8. (21 July 2006), pp. 601-603.

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Abstract

Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects.

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TY - JOUR ID - citeulike:769887 L3 - citeulike-article-id:769887 N2 - Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects. IS - 8 JF - Nature Methods SN - 1548-7091 EP - 603 TI - A unique and universal molecular barcode array PB - Nature Publishing Group VL - 3 SP - 601 KW - array KW - methodology KW - yeast AU - Pierce, Sarah AU - Fung, Eula AU - Jaramillo, Daniel AU - Chu, Angela AU - Davis, Ronald AU - Nislow, Corey AU - Giaever, Guri PY - 2006/07/21/ UR - http://dx.doi.org/10.1038/nmeth905 ER -

A unique and universal molecular barcode array Export

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