Chemical cleavage at aspartyl residues for protein identification. Export

@article{citeulike:2869413, abstract = {An alternative method to enzymatic digestion for protein identification by mass spectrometry has been developed that is based on chemical cleavage by formic acid. This method was tested on gel-purified apomyoglobin and BSA, as well as unknown proteins that cofractionate with Tyl-virus-like particles from Saccharomyces cerevisiae. Cleavage at aspartyl residues was found to be efficient and specific, and this specificity of cleavage lent itself easily to database searches. Parallel digestions using trypsin were also performed. The formic acid cleavage method generated comparable or better results than tryptic digestion for protein identification.}, address = {Protein Chemistry Laboratory, AIDS Vaccine Program, SAIC Frederick, NCI at Frederick, Maryland 21702-1201, USA. liai@ncifcrf.gov}, author = {Li, A. and Sowder, R. C. and Henderson, L. E. and Moore, S. P. and Garfinkel, D. J. and Fisher, R. J.}, citeulike-article-id = {2869413}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11816565}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11816565}, day = {15}, issn = {0003-2700}, journal = {Analytical chemistry}, keywords = {cleavage, protease, protein-analysis, proteomics}, month = {November}, number = {22}, pages = {5395--5402}, posted-at = {2008-06-06 14:26:00}, priority = {2}, title = {Chemical cleavage at aspartyl residues for protein identification.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11816565}, volume = {73}, year = {2001} }

TY - JOUR ID - citeulike:2869413 L3 - citeulike-article-id:2869413 N2 - An alternative method to enzymatic digestion for protein identification by mass spectrometry has been developed that is based on chemical cleavage by formic acid. This method was tested on gel-purified apomyoglobin and BSA, as well as unknown proteins that cofractionate with Tyl-virus-like particles from Saccharomyces cerevisiae. Cleavage at aspartyl residues was found to be efficient and specific, and this specificity of cleavage lent itself easily to database searches. Parallel digestions using trypsin were also performed. The formic acid cleavage method generated comparable or better results than tryptic digestion for protein identification. IS - 22 JF - Analytical chemistry SN - 0003-2700 AD - Protein Chemistry Laboratory, AIDS Vaccine Program, SAIC Frederick, NCI at Frederick, Maryland 21702-1201, USA. liai@ncifcrf.gov EP - 5402 TI - Chemical cleavage at aspartyl residues for protein identification. VL - 73 SP - 5395 KW - cleavage KW - protease KW - protein-analysis KW - proteomics AU - Li, A AU - Sowder, RC AU - Henderson, LE AU - Moore, SP AU - Garfinkel, DJ AU - Fisher, RJ PY - 2001/11/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/11816565 ER -