Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans. Export

@article{citeulike:4219724, abstract = {The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant wild-type and mutant CBDs from C. cellulovorans were expressed as soluble forms. Cellulose binding tests of the mutant CBDs indicated that the planar strip residues played a major role in cellulose binding and that the anchoring residues played only a minor role.}, author = {Murashima, K. and Kosugi, A. and Doi, R. H.}, citeulike-article-id = {4219724}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JB.187.20.7146-7149.2005}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16199585}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16199585}, doi = {10.1128/JB.187.20.7146-7149.2005}, issn = {0021-9193}, journal = {Journal of bacteriology}, keywords = {ecoli, expression, mbp}, month = {October}, number = {20}, pages = {7146--7149}, posted-at = {2009-03-25 14:43:29}, priority = {2}, title = {Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans.}, url = {http://dx.doi.org/10.1128/JB.187.20.7146-7149.2005}, volume = {187}, year = {2005} }

TY - JOUR ID - citeulike:4219724 L3 - citeulike-article-id:4219724 N2 - The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant wild-type and mutant CBDs from C. cellulovorans were expressed as soluble forms. Cellulose binding tests of the mutant CBDs indicated that the planar strip residues played a major role in cellulose binding and that the anchoring residues played only a minor role. IS - 20 JF - Journal of bacteriology SN - 0021-9193 EP - 7149 TI - Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans. VL - 187 SP - 7146 KW - ecoli KW - expression KW - mbp AU - Murashima, K AU - Kosugi, A AU - Doi, RH PY - 2005/10// UR - http://dx.doi.org/10.1128/JB.187.20.7146-7149.2005 ER -