Amino acid analysis at the picomole level. Application to the C-terminal sequence analysis of polypeptides. Export

@article{citeulike:4270331, abstract = {Amino acids labelled with dimethylaminoazobenzenesulphonyl chloride can be separated by reversed-phase high-pressure liquid chromatography and detected in the visible region (436 nm). All 19 naturally occurring amino acids can be separated on a Zorbax ODS column by employing two different gradient systems consisting of an acetonitrile/aqueous buffer mixture. As little as 2--5 pmol of an individual dimethylaminoazobenzenesulphonyl-amino acid can be quantitatively analysed with reliability, and only 10--30 ng of the dimethylaminoazobenzenesulphonylated protein hydrolysate is needed for each complete amino acid analysis. This new technique is as sensitive as any of the current amino acid analysis methods involving ion-exchange separation plus fluorescence detection, and is technically much simpler. By the combination of this sensitive amino acid-analysing technique with carboxypeptidase, we have been able to determine the C-terminal sequence of polypeptides at the picomole level.}, author = {Chang, J. Y. and Knecht, R. and Braun, D. G.}, citeulike-article-id = {4270331}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/6803769}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=6803769}, day = {1}, issn = {0264-6021}, journal = {The Biochemical journal}, keywords = {aminoacid, aminoacid-analysis, analysis, dabsylation, hplc, labeling}, month = {December}, number = {3}, pages = {547--555}, posted-at = {2009-04-03 18:48:49}, priority = {2}, title = {Amino acid analysis at the picomole level. Application to the C-terminal sequence analysis of polypeptides.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/6803769}, volume = {199}, year = {1981} }

TY - JOUR ID - citeulike:4270331 L3 - citeulike-article-id:4270331 N2 - Amino acids labelled with dimethylaminoazobenzenesulphonyl chloride can be separated by reversed-phase high-pressure liquid chromatography and detected in the visible region (436 nm). All 19 naturally occurring amino acids can be separated on a Zorbax ODS column by employing two different gradient systems consisting of an acetonitrile/aqueous buffer mixture. As little as 2--5 pmol of an individual dimethylaminoazobenzenesulphonyl-amino acid can be quantitatively analysed with reliability, and only 10--30 ng of the dimethylaminoazobenzenesulphonylated protein hydrolysate is needed for each complete amino acid analysis. This new technique is as sensitive as any of the current amino acid analysis methods involving ion-exchange separation plus fluorescence detection, and is technically much simpler. By the combination of this sensitive amino acid-analysing technique with carboxypeptidase, we have been able to determine the C-terminal sequence of polypeptides at the picomole level. IS - 3 JF - The Biochemical journal SN - 0264-6021 EP - 555 TI - Amino acid analysis at the picomole level. Application to the C-terminal sequence analysis of polypeptides. VL - 199 SP - 547 KW - aminoacid KW - aminoacid-analysis KW - analysis KW - dabsylation KW - hplc KW - labeling AU - Chang, JY AU - Knecht, R AU - Braun, DG PY - 1981/12/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/6803769 ER -