Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10. Export

@article{citeulike:504095, abstract = {A Comamonas acidovorans strain, designated NBA-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. When grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. In cell extracts with NADPH present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. Partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-nitrosobenzoate to 4-hydroxylamino-benzoate. The substrate specificity of the enzyme was narrow and NADPH was 15 times more effective as a cofactor than NADH. The results provide evidence for a novel pathway for aerobic degradation of 4-nitrobenzoate, since neither 4-hydroxybenzoate nor 4-aminobenzoate were involved in the degradative pathway.}, address = {Department of Food Science, Agricultural University, Wageningen, The Netherlands.}, author = {Groenewegen, P. E. and Breeuwer, P. and van Helvoort, J. M. and Langenhoff, A. A. and de Vries, F. P. and de Bont, J. A.}, citeulike-article-id = {504095}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/1527502}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=1527502}, issn = {0022-1287}, journal = {J Gen Microbiol}, keywords = {aromatic-nitro, bio-degradation, hydroxylamine}, month = {August}, pages = {1599--1605}, posted-at = {2006-02-13 14:39:51}, priority = {2}, title = {Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1527502}, volume = {138 Pt 8}, year = {1992} }

TY - JOUR ID - citeulike:504095 L3 - citeulike-article-id:504095 N2 - A Comamonas acidovorans strain, designated NBA-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. When grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. In cell extracts with NADPH present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. Partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-nitrosobenzoate to 4-hydroxylamino-benzoate. The substrate specificity of the enzyme was narrow and NADPH was 15 times more effective as a cofactor than NADH. The results provide evidence for a novel pathway for aerobic degradation of 4-nitrobenzoate, since neither 4-hydroxybenzoate nor 4-aminobenzoate were involved in the degradative pathway. JF - J Gen Microbiol SN - 0022-1287 AD - Department of Food Science, Agricultural University, Wageningen, The Netherlands. EP - 1605 TI - Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10. VL - 138 Pt 8 SP - 1599 KW - aromatic-nitro KW - bio-degradation KW - hydroxylamine AU - Groenewegen, PE AU - Breeuwer, P AU - van Helvoort, JM AU - Langenhoff, AA AU - de Vries, FP AU - de Bont, JA PY - 1992/08// UR - http://view.ncbi.nlm.nih.gov/pubmed/1527502 ER -