A novel pathway for the catabolism of 4-nitrotoluene by Pseudomonas. Export

@article{citeulike:504115, abstract = {Eleven strains of Pseudomonas were isolated by selective enrichment on 4-nitrotoluene (4NT). They all utilized 4NT, 4-nitrobenzyl alcohol (4NBA) or 4-nitrobenzoate (4NBZate) as sole sources of carbon and nitrogen. One strain, TW3, was used for more detailed studies. 4NT-grown cells of TW3 take up O2 when incubated in the presence of 4NBA, 4-nitrobenzaldehyde (4NBZ) and 4NBZate. HPLC analysis of culture supernatants showed that 4NBZ and 4NBZate were formed when 4NT-grown cells wer incubated with 4NBA, whereas only 4NBZate was found when they were incubated with 4NBZ. Two dehydrogenases were detected in extracts of 4NT-grown cells. 4NBA dehydrogenase could be assayed by a dye-linked assay whereas 4NBZ dehydrogenase activity was linked to NAD+ reduction. No nitrite was detected in supernatants of 4NBZate-grown cells incubated with 4NBZate but the nitrogen appeared as ammonium. The only aromatic ring-cleavage dioxygenase that was induced during growth on the nitroaromatics was protocatechuate 3,4-dioxygenase. It is proposed that the pathway for 4NT catabolism proceeds via 4NBA, 4NBZ and 4NBZate and ultimately to protocatechuate with release of the nitro group as ammonium.}, address = {School of Biological Sciences, University of Wales, Bangor, Gwynedd, UK.}, author = {Rhys-Williams, W. and Taylor, S. C. and Williams, P. A.}, citeulike-article-id = {504115}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8245826}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8245826}, issn = {0022-1287}, journal = {J Gen Microbiol}, keywords = {aromatic-nitro, bio-degradation}, month = {September}, number = {9}, pages = {1967--1972}, posted-at = {2006-02-13 15:28:01}, priority = {2}, title = {A novel pathway for the catabolism of 4-nitrotoluene by Pseudomonas.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8245826}, volume = {139}, year = {1993} }

TY - JOUR ID - citeulike:504115 L3 - citeulike-article-id:504115 N2 - Eleven strains of Pseudomonas were isolated by selective enrichment on 4-nitrotoluene (4NT). They all utilized 4NT, 4-nitrobenzyl alcohol (4NBA) or 4-nitrobenzoate (4NBZate) as sole sources of carbon and nitrogen. One strain, TW3, was used for more detailed studies. 4NT-grown cells of TW3 take up O2 when incubated in the presence of 4NBA, 4-nitrobenzaldehyde (4NBZ) and 4NBZate. HPLC analysis of culture supernatants showed that 4NBZ and 4NBZate were formed when 4NT-grown cells wer incubated with 4NBA, whereas only 4NBZate was found when they were incubated with 4NBZ. Two dehydrogenases were detected in extracts of 4NT-grown cells. 4NBA dehydrogenase could be assayed by a dye-linked assay whereas 4NBZ dehydrogenase activity was linked to NAD+ reduction. No nitrite was detected in supernatants of 4NBZate-grown cells incubated with 4NBZate but the nitrogen appeared as ammonium. The only aromatic ring-cleavage dioxygenase that was induced during growth on the nitroaromatics was protocatechuate 3,4-dioxygenase. It is proposed that the pathway for 4NT catabolism proceeds via 4NBA, 4NBZ and 4NBZate and ultimately to protocatechuate with release of the nitro group as ammonium. IS - 9 JF - J Gen Microbiol SN - 0022-1287 AD - School of Biological Sciences, University of Wales, Bangor, Gwynedd, UK. EP - 1972 TI - A novel pathway for the catabolism of 4-nitrotoluene by Pseudomonas. VL - 139 SP - 1967 KW - aromatic-nitro KW - bio-degradation AU - Rhys-Williams, W AU - Taylor, SC AU - Williams, PA PY - 1993/09// UR - http://view.ncbi.nlm.nih.gov/pubmed/8245826 ER -