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robert's expression [43 articles]

Recent papers added to robert's library classified by the tag expression. You can also see everyone's expression.
  • Controlled intracellular processing of fusion proteins by TEV protease.
    Protein expression and purification, Vol. 19, No. 2. (July 2000), pp. 312-318.
    by RB Kapust, DS Waugh
    posted to cleavage ecoli expression protease purification by robert on 2008-04-15 15:47:09 as **
  • Processive degradation of nascent polypeptides, triggered by tandem AGA codons, limits the accumulation of recombinant tobacco etch virus protease in Escherichia coli BL21(DE3).
    Protein expression and purification, Vol. 24, No. 1. (February 2002), pp. 61-70.
    by RB Kapust, KM Routzahn, DS Waugh
    posted to cleavage cloning ecoli expression protease by robert on 2008-04-15 15:46:12 as **
  • A yeast-based growth assay for the analysis of site-specific proteases.
    Nucleic acids research, Vol. 31, No. 4. (15 February 2003)
    by F Köhler
    posted to cleavage ecoli expression protease screening by robert on 2008-04-15 15:42:35 as **
  • Inhibition of tobacco etch virus protease activity by detergents.
    Protein expression and purification, Vol. 27, No. 1. (January 2003), pp. 109-114.
    by AK Mohanty, CR Simmons, MC Wiener
    posted to cleavage cloning ecoli expression by robert on 2008-04-15 15:41:41 as **
  • Structural basis for the substrate specificity of tobacco etch virus protease.
    The Journal of biological chemistry, Vol. 277, No. 52. (27 December 2002), pp. 50564-50572.
    by J Phan, A Zdanov, AG Evdokimov, JE Tropea, HK Peters, RB Kapust, M Li, A Wlodawer, DS Waugh
    posted to cleavage cloning ecoli expression structure-function by robert on 2008-04-15 15:40:23 as **
  • Overnight ExpressTM Autoinduction Systems
    (2005)
    by Company Novagen
    posted to ecoli expression by robert on 2008-04-07 14:54:20 as **
  • Protein production by auto-induction in high density shaking cultures.
    Protein Expr Purif, Vol. 41, No. 1. (May 2005), pp. 207-234.
    by FW Studier
    posted to ecoli expression by robert on 2008-04-07 14:04:13 as ** along with 4 people becarmson martainn jrweir82 min013
  • Protein Production by Auto-Induction in High-Density Shaking Cultures
    (20 December 2007)
    by William F Studier
    posted to ecoli expression by robert on 2008-04-07 14:03:19 as **
  • T7 Promoter Based Prokaryotic Expression Systems
    by Internetseite Invitrogen
    posted to ecoli expression by robert on 2008-04-03 17:28:03 as **
  • Structural characterization of in vitro and in vivo intermediates on the loading module of microcystin synthetase.
    ACS chemical biology, Vol. 1, No. 2. (17 March 2006), pp. 93-102.
    by LM Hicks, MC Moffitt, LL Beer, BS Moore, NL Kelleher
    posted to biosynthesis expression pptase by robert on 2008-04-02 09:01:14 as **
  • The QIAexpressionist
    (June 2003)
    by Company Qiagen
    posted to ecoli expression by robert on 2008-04-01 12:02:26 as **
  • A dual expression platform to optimize the soluble production of heterologous proteins in the periplasm of Escherichia coli
    Applied Microbiology and Biotechnology, Vol. 76, No. 6. (October 2007), pp. 1413-1422.
    by Kraft, Mario, Knupfer, Uwe, Wenderoth, Rolf, Kacholdt, Andre, Pietschmann, Patricia, Hock, Bjorn, Horn, Uwe
    posted to ecoli expression by robert on 2008-04-01 11:37:35 as **
  • Duale Optimierung der Darstellung heterologer Proteine in Escherichia coli: Parallele und differenzielle Produktion von Centromerproteinen des inneren humanen Kinetochors
    (2007)
    by Mario Kraft
    posted to ecoli expression by robert on 2008-04-01 11:34:08 as **
  • Efficient gene synthesis by Klenow assembly/extension-Pfu polymerase amplification (KAPPA) of overlapping oligonucleotides.
    PCR Methods Appl, Vol. 4, No. 5. (April 1995), pp. 299-302.
    by EW Holowachuk, MS Ruhoff
    posted to expression pcr by robert on 2007-08-22 10:22:40 as **
  • Two-step total gene synthesis method.
    Nucleic Acids Res, Vol. 32, No. 7. (2004)
    by L Young, Q Dong
    posted to codon-usage expression pcr by robert on 2007-08-22 10:15:52 as **
  • Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus.
    FEMS Microbiol Lett, Vol. 216, No. 2. (5 November 2002), pp. 179-183.
    by M Ishida, T Oshima, K Yutani
    posted to cloning codon-usage ecoli expression by robert on 2006-10-16 09:35:54 as **
  • High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions.
    Appl Microbiol Biotechnol, Vol. 46, No. 5-6. (December 1996), pp. 524-532.
    by U Horn, W Strittmatter, A Krebber, U Knüpfer, M Kujau, R Wenderoth, K Müller, S Matzku, A Plückthun, D Riesenberg
    posted to ecoli expression by robert on 2006-07-19 15:32:06 as **
  • pRSET A,B,C
    (2006)
    by Internetseite Invitrogen
    posted to cloning expression fusion by robert on 2006-05-23 13:39:55 as **
  • BL21(DE3) Competent Cells
    (2006)
    by Manual Stratagene
    posted to ecoli expression by robert on 2006-05-08 16:10:51 as **
  • Site-specific degradation of Streptomyces lividans DNA during electrophoresis in buffers contaminated with ferrous iron.
    Nucleic Acids Res, Vol. 16, No. 10. (25 May 1988), pp. 4341-4352.
    by X Zhou, Z Deng, JL Firmin, DA Hopwood, T Kieser
    posted to cloning expression streptomyces by robert on 2006-05-08 10:49:19 as **
  • Genetic transformation of Streptomyces globisporus strain 1912: Restriction barrier and plasmid compatibility
    Mikrobiol Z, Vol. 63, No. 1. (b 2001), pp. 15-22.
    by AB Matseliukh
    posted to cloning expression streptomyces by robert on 2006-05-08 09:55:10 as **
  • Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.
    Protein Eng, Vol. 14, No. 12. (December 2001), pp. 993-1000.
    by RB Kapust, J Tözsér, JD Fox, DE Anderson, S Cherry, TD Copeland, DS Waugh
    posted to cleavage cloning expression fusion protease by robert on 2006-02-27 10:18:18 as **
  • The P1' specificity of tobacco etch virus protease.
    Biochem Biophys Res Commun, Vol. 294, No. 5. (28 June 2002), pp. 949-955.
    by RB Kapust, J Tözsér, TD Copeland, DS Waugh
    posted to cleavage expression fusion protease by robert on 2006-02-27 10:17:11 as **
  • Crystal structures of fusion proteins with large-affinity tags.
    Protein Sci, Vol. 12, No. 7. (July 2003), pp. 1313-1322.
    by DR Smyth, MK Mrozkiewicz, WJ McGrath, P Listwan, B Kobe
    posted to crystal-structure expression fusion by robert on 2006-02-24 15:07:00 as **
  • De novo design of an IL-4 antagonist and its structure at 1.9 A.
    Proc Natl Acad Sci U S A, Vol. 102, No. 6. (8 February 2005), pp. 1889-1894.
    by SL Laporte, CM Forsyth, BC Cunningham, LJ Miercke, D Akhavan, RM Stroud
    posted to crystal-structure expression by robert on 2006-02-24 15:05:19 as **
  • pWHM4* vector map
    by Hki Inhouse
    posted to cloning expression streptomyces by robert on 2006-02-09 09:53:59 as **
  • pGEM T and pGEM T easy
    by Internetseite Promega
    posted to affinity cloning ecoli expression fusion by robert on 2006-02-09 09:51:10 as **
  • New England Biolabs, DNA Sequences and Maps
    by Internetseite Neb
    posted to affinity cloning ecoli expression fusion by robert on 2006-02-09 09:37:12 as **
  • Codon usage tabulated from international DNA sequence databases: status for the year 2000.
    Nucleic Acids Res, Vol. 28, No. 1. (1 January 2000)
    by Y Nakamura, T Gojobori, T Ikemura
    posted to codon-usage ecoli expression by robert on 2006-02-09 09:15:50 as ** along with 1 person nagendraproj
  • Posttranslational processing of the xylanase Xys1L from Streptomyces halstedii JM8 is carried out by secreted serine proteases.
    Microbiology, Vol. 149, No. Pt 7. (July 2003), pp. 1623-1632.
    by JM Fernandez-Abalos, V Reviejo, M Diaz, S Rodriguez, F Leal, RI Santamaria
    posted to expression posttanslation-processing streptomyces by robert on 2006-02-08 15:03:06 as **
  • A glutamate mutase is involved in the biosynthesis of the lipopeptide antibiotic friulimicin in Actinoplanes friuliensis.
    Antimicrob Agents Chemother, Vol. 47, No. 2. (February 2003), pp. 447-457.
    by E Heinzelmann, S Berger, O Puk, B Reichenstein, W Wohlleben, D Schwartz
    posted to biosynthesis expression histag streptomyces by robert on 2006-02-08 15:02:00 as ** along with 1 person manzanita
  • Identification of a topoisomerase IV in actinobacteria: purification and characterization of ParYR and GyrBR from the coumermycin A1 producer Streptomyces rishiriensis DSM 40489.
    Microbiology, Vol. 150, No. Pt 3. (March 2004), pp. 641-647.
    by E Schmutz, S Hennig, SM Li, L Heide
    posted to biosynthesis cloning expression streptomyces by robert on 2006-02-08 14:56:43 as **
  • Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus).
    J Bacteriol, Vol. 171, No. 11. (November 1989), pp. 5872-5881.
    by J Vara, M Lewandowska-Skarbek, YG Wang, S Donadio, CR Hutchinson
    posted to biosynthesis cloning expression pwhm streptomyces by robert on 2006-02-08 14:54:58 as **
  • Streptomyces lividans as a host for the production and secretion of Escherichia coli TEM beta-lactamase.
    Lett Appl Microbiol, Vol. 28, No. 4. (April 1999), pp. 321-326.
    by C Isiegas, V Parro, RP Mellado
    posted to ecoli expression streptomyces by robert on 2006-02-08 14:52:20 as **
  • Heterologous biopharmaceutical protein expression in Streptomyces.
    Trends Biotechnol, Vol. 15, No. 8. (August 1997), pp. 315-320.
    by C Binnie, JD Cossar, DI Stewart
    posted to expression streptomyces by robert on 2006-02-08 14:51:13 as **
  • BL21-CodonPlus Competent Cells
    (2005)
    by Manual Stratagene
    posted to competent-cells expression by robert on 2006-02-08 13:01:34 as **
  • Competent Cells
    (2006)
    by Manual Novagen
    posted to competent-cells expression by robert on 2006-02-08 13:00:32 as **
  • Host Strain Competent Cell Selection Guide
    by Manual Novagen
    posted to competent-cells expression by robert on 2006-02-08 12:58:55 as **
  • Enhanced heterologous expression of two Streptomyces griseolus cytochrome P450s and Streptomyces coelicolor ferredoxin reductase as potentially efficient hydroxylation catalysts.
    Appl Environ Microbiol, Vol. 69, No. 1. (January 2003), pp. 373-382.
    by HA Hussain, JM Ward
    posted to ecoli expression ferredoxin p450 streptomyces by robert on 2006-02-08 11:56:27 as **
  • The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm.
    J Biol Chem, Vol. 272, No. 25. (20 June 1997), pp. 15661-15667.
    by WA Prinz, F Aslund, A Holmgren, J Beckwith
    posted to ecoli expression by robert on 2006-02-08 11:54:03 as ** along with 1 person Gehrke
  • Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins.
    Protein Expr Purif, Vol. 11, No. 1. (October 1997), pp. 1-16.
    by J Nilsson, S Ståhl, J Lundeberg, M Uhlén, PA Nygren
    posted to affinity detection expression fusion immobilisation purification by robert on 2006-02-08 11:45:10 as **
  • Chaperonins govern growth of Escherichia coli at low temperatures.
    Nat Biotechnol, Vol. 21, No. 11. (November 2003), pp. 1266-1267.
    by M Ferrer, TN Chernikova, MM Yakimov, PN Golyshin, KN Timmis
    posted to ecoli expression by robert on 2006-02-08 11:40:34 as **
  • Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.
    Biotechnol Appl Biochem, Vol. 35, No. Pt 2. (April 2002), pp. 91-105.
    by P Jonasson, S Liljeqvist, Pa Nygren, S Stahl
    posted to affinity ecoli expression fusion purification by robert on 2006-02-08 11:37:19 as **
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