Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. Export

@article{citeulike:4935798, abstract = {The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity.}, author = {Beltrao, Pedro and Trinidad, Jonathan C. and Fiedler, Dorothea and Roguev, Assen and Lim, Wendell A. and Shokat, Kevan M. and Burlingame, Alma L. and Krogan, Nevan J.}, citeulike-article-id = {4935798}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pbio.1000134}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19547744}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19547744}, day = {16}, doi = {10.1371/journal.pbio.1000134}, issn = {1545-7885}, journal = {PLoS Biol}, keywords = {biological, evolution, large, network, phosphorylation, regulation, scale, yeast}, month = {Jun}, number = {6}, pages = {e1000134+}, posted-at = {2009-08-03 18:34:54}, priority = {2}, publisher = {Public Library of Science}, title = {Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.}, url = {http://dx.doi.org/10.1371/journal.pbio.1000134}, volume = {7}, year = {2009} }

TY - JOUR ID - citeulike:4935798 L3 - citeulike-article-id:4935798 N2 - The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity. IS - 6 JF - PLoS Biol SN - 1545-7885 TI - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. PB - Public Library of Science VL - 7 SP - e1000134 KW - biological KW - evolution KW - large KW - network KW - phosphorylation KW - regulation KW - scale KW - yeast AU - Beltrao, Pedro AU - Trinidad, Jonathan AU - Fiedler, Dorothea AU - Roguev, Assen AU - Lim, Wendell AU - Shokat, Kevan AU - Burlingame, Alma AU - Krogan, Nevan PY - 2009/Jun/16/ UR - http://dx.doi.org/10.1371/journal.pbio.1000134 ER -