Differential Regulation of Horizontally Acquired and Core Genome Genes by the Bacterial Modulator H-NS Export

@article{citeulike:5372965, abstract = {Author Summary Acquisition of DNA by horizontal gene transfer (HGT) significantly increases bacterial genetic variability. Relevant issues are the mechanisms that bacterial cells have evolved to efficiently integrate the newly acquired DNA into the host cell regulatory machinery. In Gram negative cells, the nucleoid associated protein H-NS has been shown to bind AT-rich sequences of HGT DNA and silence unwanted expression of these genes. This has led to consider H-NS as a "genome sentinel." Nevertheless, this proposed role must be compatible with its role modulating core genome genes. Weak expression of recently transferred genes must be coordinated with proper expression levels of housekeeping genes. In this paper, we describe a strategy that enteric bacteria have developed to differentially modulate HGT and core genome genes. Two independent lines of experimental evidence suggest that the H-NS system of enteric bacteria may have evolved to discriminate between core genome and HGT DNA. The plasmid R27-encoded H-NS protein selectively modulates HGT genes. This avoids plasmid-encoded H-NS interfering with modulation of core functions. We also show that, for efficient silencing of HGT genes, resident chromosomal H-NS recruits the Hha protein and forms heteromeric complexes with DNA. In contrast, housekeeping genes are modulated by H-NS alone.}, author = {Nos, Rosa C. and Vivero, Aitziber and Aznar, Sonia and Garc\'{\i}a, Jes\'{u}s and Pons, Miquel and Madrid, Cristina and Ju\'{a}rez, Antonio}, citeulike-article-id = {5372965}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pgen.1000513}, day = {12}, doi = {10.1371/journal.pgen.1000513}, journal = {PLoS Genet}, keywords = {elements, gene, horizontal, mobile, regulation, transcription, transfer}, month = {Jun}, number = {6}, pages = {e1000513+}, posted-at = {2009-08-05 16:59:56}, priority = {2}, publisher = {Public Library of Science}, title = {Differential Regulation of Horizontally Acquired and Core Genome Genes by the Bacterial Modulator H-NS}, url = {http://dx.doi.org/10.1371/journal.pgen.1000513}, volume = {5}, year = {2009} }

TY - JOUR ID - citeulike:5372965 L3 - citeulike-article-id:5372965 N2 - Author Summary Acquisition of DNA by horizontal gene transfer (HGT) significantly increases bacterial genetic variability. Relevant issues are the mechanisms that bacterial cells have evolved to efficiently integrate the newly acquired DNA into the host cell regulatory machinery. In Gram negative cells, the nucleoid associated protein H-NS has been shown to bind AT-rich sequences of HGT DNA and silence unwanted expression of these genes. This has led to consider H-NS as a "genome sentinel." Nevertheless, this proposed role must be compatible with its role modulating core genome genes. Weak expression of recently transferred genes must be coordinated with proper expression levels of housekeeping genes. In this paper, we describe a strategy that enteric bacteria have developed to differentially modulate HGT and core genome genes. Two independent lines of experimental evidence suggest that the H-NS system of enteric bacteria may have evolved to discriminate between core genome and HGT DNA. The plasmid R27-encoded H-NS protein selectively modulates HGT genes. This avoids plasmid-encoded H-NS interfering with modulation of core functions. We also show that, for efficient silencing of HGT genes, resident chromosomal H-NS recruits the Hha protein and forms heteromeric complexes with DNA. In contrast, housekeeping genes are modulated by H-NS alone. IS - 6 JF - PLoS Genet TI - Differential Regulation of Horizontally Acquired and Core Genome Genes by the Bacterial Modulator H-NS PB - Public Library of Science VL - 5 SP - e1000513 KW - elements KW - gene KW - horizontal KW - mobile KW - regulation KW - transcription KW - transfer AU - Nos, Rosa AU - Vivero, Aitziber AU - Aznar, Sonia AU - García, Jesús AU - Pons, Miquel AU - Madrid, Cristina AU - Juárez, Antonio PY - 2009/Jun/12/ UR - http://dx.doi.org/10.1371/journal.pgen.1000513 ER -