Lock-in by molecular multiplication Export

@article{citeulike:1496192, abstract = {A lock-in amplifier is physically realized at the level of fluorescent dye molecules. It is based on the general property that the emission of a fluorescent dye is the product of quantum efficiency and illumination intensity. For each pixel of a microscopic image, we measure in amplitude and phase an environment property of the dye, such as conformation, membrane voltage, or temperature. This lock-in implementation is highly parallel and reaches the ultimate photon shot noise limit. Using fast temperature oscillations, we apply it to measure the opening/closing kinetics of a molecular beacon (DNA hairpin) at 5 \µs resolution. \©2003 American Institute of Physics.}, author = {Braun, Dieter and Libchaber, Albert}, citeulike-article-id = {1496192}, citeulike-linkout-0 = {http://scitation.aip.org/getabs/servlet/GetabsServlet?prog=normal&id=APPLAB000083000026005554000001&idtype=cvips&gifs=yes}, citeulike-linkout-1 = {http://link.aip.org/link/?APL/83/5554}, citeulike-linkout-2 = {http://dx.doi.org/10.1063/1.1629782}, doi = {10.1063/1.1629782}, journal = {Applied Physics Letters}, keywords = {amplitude, beacon, dna, efficiency, florescence, hairpin, heat, infrared, led, molecular, oscillations, phase, quantum, temperature, theory}, number = {26}, pages = {5554--5556}, posted-at = {2007-07-26 15:45:37}, priority = {0}, publisher = {AIP}, title = {Lock-in by molecular multiplication}, url = {http://dx.doi.org/10.1063/1.1629782}, volume = {83}, year = {2003} }

TY - JOUR ID - citeulike:1496192 L3 - citeulike-article-id:1496192 N2 - A lock-in amplifier is physically realized at the level of fluorescent dye molecules. It is based on the general property that the emission of a fluorescent dye is the product of quantum efficiency and illumination intensity. For each pixel of a microscopic image, we measure in amplitude and phase an environment property of the dye, such as conformation, membrane voltage, or temperature. This lock-in implementation is highly parallel and reaches the ultimate photon shot noise limit. Using fast temperature oscillations, we apply it to measure the opening/closing kinetics of a molecular beacon (DNA hairpin) at 5 µs resolution. ©2003 American Institute of Physics. IS - 26 JF - Applied Physics Letters EP - 5556 TI - Lock-in by molecular multiplication PB - AIP VL - 83 SP - 5554 KW - amplitude KW - beacon KW - dna KW - efficiency KW - florescence KW - hairpin KW - heat KW - infrared KW - led KW - molecular KW - oscillations KW - phase KW - quantum KW - temperature KW - theory AU - Braun, Dieter AU - Libchaber, Albert PY - 2003/// UR - http://dx.doi.org/10.1063/1.1629782 ER -