Ceramic-Based Nanoparticles Entrapping Water-Insoluble Photosensitizing Anticancer Drugs: A Novel Drug-Carrier System for Photodynamic Therapy Export

@article{citeulike:1545054, abstract = {Abstract: A novel nanoparticle-based drug carrier for photodynamic therapy is reported which can provide stable aqueous dispersion of hydrophobic photosensitizers, yet preserve the key step of photogeneration of singlet oxygen, necessary for photodynamic action. A multidisciplinary approach is utilized which involves (i) nanochemistry in micellar cavity to produce these carriers, (ii) spectroscopy to confirm singlet oxygen production, and (iii) in vitro studies using tumor cells to investigate drug-carrier uptake and destruction of cancer cells by photodynamic action. Ultrafine organically modified silica-based nanoparticles (diameter \~{}30 nm), entrapping water-insoluble photosensitizing anticancer drug 2-devinyl-2-(1-hexyloxyethyl) pyropheophorbide, have been synthesized in the nonpolar core of micelles by hydrolysis of triethoxyvinylsilane. The resulting drug-doped nanoparticles are spherical, highly monodispersed, and stable in aqueous system. The entrapped drug is more fluorescent in aqueous medium than the free drug, permitting use of fluorescence bioimaging studies. Irradiation of the photosensitizing drug entrapped in nanoparticles with light of suitable wavelength results in efficient generation of singlet oxygen, which is made possible by the inherent porosity of the nanoparticles. In vitro studies have demonstrated the active uptake of drug-doped nanoparticles into the cytosol of tumor cells. Significant damage to such impregnated tumor cells was observed upon irradiation with light of wavelength 650 nm. Thus, the potential of using ceramic-based nanoparticles as drug carriers for photodynamic therapy has been demonstrated.}, address = {Contribution from the Institute of Lasers, Photonics and Biophotonics, Department of Chemistry, State University of New York, Buffalo, New York 14260-3000, and Department of Dermatology and Photodynamic Therapy Center, Roswell Park Cancer Institute, Buffalo, New York 14263}, author = {Roy, I. and Ohulchanskyy, T. Y. and Pudavar, H. E. and Bergey, E. J. and Oseroff, A. R. and Morgan, J. and Dougherty, T. J. and Prasad, P. N.}, citeulike-article-id = {1545054}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja0343095}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja0343095}, day = {2}, doi = {10.1021/ja0343095}, journal = {J. Am. Chem. Soc.}, keywords = {anticancer, ceramic, drug-carrier, nanoparticles, pdt, photodynamic, photosensitizers, therapy}, month = {July}, number = {26}, pages = {7860--7865}, posted-at = {2007-08-09 06:03:09}, priority = {2}, title = {Ceramic-Based Nanoparticles Entrapping Water-Insoluble Photosensitizing Anticancer Drugs: A Novel Drug-Carrier System for Photodynamic Therapy}, url = {http://dx.doi.org/10.1021/ja0343095}, volume = {125}, year = {2003} }

TY - JOUR ID - citeulike:1545054 L3 - citeulike-article-id:1545054 N2 - Abstract: A novel nanoparticle-based drug carrier for photodynamic therapy is reported which can provide stable aqueous dispersion of hydrophobic photosensitizers, yet preserve the key step of photogeneration of singlet oxygen, necessary for photodynamic action. A multidisciplinary approach is utilized which involves (i) nanochemistry in micellar cavity to produce these carriers, (ii) spectroscopy to confirm singlet oxygen production, and (iii) in vitro studies using tumor cells to investigate drug-carrier uptake and destruction of cancer cells by photodynamic action. Ultrafine organically modified silica-based nanoparticles (diameter ~30 nm), entrapping water-insoluble photosensitizing anticancer drug 2-devinyl-2-(1-hexyloxyethyl) pyropheophorbide, have been synthesized in the nonpolar core of micelles by hydrolysis of triethoxyvinylsilane. The resulting drug-doped nanoparticles are spherical, highly monodispersed, and stable in aqueous system. The entrapped drug is more fluorescent in aqueous medium than the free drug, permitting use of fluorescence bioimaging studies. Irradiation of the photosensitizing drug entrapped in nanoparticles with light of suitable wavelength results in efficient generation of singlet oxygen, which is made possible by the inherent porosity of the nanoparticles. In vitro studies have demonstrated the active uptake of drug-doped nanoparticles into the cytosol of tumor cells. Significant damage to such impregnated tumor cells was observed upon irradiation with light of wavelength 650 nm. Thus, the potential of using ceramic-based nanoparticles as drug carriers for photodynamic therapy has been demonstrated. IS - 26 JF - J. Am. Chem. Soc. AD - Contribution from the Institute of Lasers, Photonics and Biophotonics, Department of Chemistry, State University of New York, Buffalo, New York 14260-3000, and Department of Dermatology and Photodynamic Therapy Center, Roswell Park Cancer Institute, Buffalo, New York 14263 EP - 7865 TI - Ceramic-Based Nanoparticles Entrapping Water-Insoluble Photosensitizing Anticancer Drugs: A Novel Drug-Carrier System for Photodynamic Therapy VL - 125 SP - 7860 KW - anticancer KW - ceramic KW - drug-carrier KW - nanoparticles KW - pdt KW - photodynamic KW - photosensitizers KW - therapy AU - Roy, I AU - Ohulchanskyy, TY AU - Pudavar, HE AU - Bergey, EJ AU - Oseroff, AR AU - Morgan, J AU - Dougherty, TJ AU - Prasad, PN PY - 2003/07/02/ UR - http://dx.doi.org/10.1021/ja0343095 ER -