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Cell and organ printing 1: protein and cell printers.by: W. C. Wilson, T. Boland
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AbstractWe have developed several devices for positioning organic molecules, molecular aggregates, cells, and single-cell organisms onto solid supports. These printers can create stable, functional protein arrays using an inexpensive technology. The cell printer allows us to create cell libraries as well as cellular assemblies that mimic their respective position in organs. The printers are derived from commercially available ink-jet printers that are modified to dispense protein or cell solutions instead of ink. We describe here the modifications to the print heads, and the printer hardware and software that enabled us to adapt the ink-jet printers for the manufacture of cell and protein arrays. The printers have the advantage of being fully automated and computer controlled, and allow for the high-throughput manufacture of protein and cell arrays.
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RIS record1268nm 193nm 1-d 1-photon 2-d 3-d 3-mi 567nm 5th aberrations ablation absolute absorption acetate acetone acetonitrile achromatic acids acoustic adaptive adhesion adsorption aerosols afm ag agents agglomerates aggregates air alcohol algaas algorithms alkanethiol amorphous amplification amplified amplifier amplitude analysis angle angles angular animal anisotropic anisotropy annealing annular anomalous antennae anthracene antibacterial antibodies anticancer antigen antimony anti-stokes ao_2002 aperture apertureless aqueous arbitrary archimedes arrays arsenide artificial arxiv aspect-ratio aspherical assay assembly asymmetric asynchronous atherosclerotic atom atomic atoms attenuation attomolar attosecond au autocorrelation avidin avogadro axial axicon axisymmetric axoneme azobenzene az-pf514 back-focal-plane background bacteria bacterial bacteriophage band3 bandages bandgaps barium base bath beacon beads beam beamsplitter benzothiadiazole bfp biased bifurcation bilayer bimetallic binding bioassays biocavity biochemistry biocompatibility biodegradable biodiagnostics bioeliminable biological biology biomechanics biomedical biomimetic biomimicry bioparticles biophysics biosensors biotechnology biotin biotribology biphasic bipolar bispheres bleach block blood blue boltzmann book boron bottle boundary bowtie bragg breakdown breast brownian bsa bubbles buckling c ca cadmium cage calcium calculus calibration cancer capacitance capacity capillary capped capsids capture carbide carbon career carlo catalysis cations causality caveat cavitation cavity ccd cd cds cdte cell cells centimeter centroid ceramic chain chains chaos characterization charge chemical chemistry chemotaxis chiral cholate chromatin chromium chromophores chromosomes circuits circular citeby2 citedby1 citedby2 citedby3 citedby4 citedby5 citedby6 citedby7 cleavable click clusters cns co coatings coaxial cobalt code coefficient coherence coil cold coli collapse collective collisional colloidal color commensurate comment commercial community comparator compensation complex composites computed computing concentration condensed conductivity confocal conformational conical conjugate conservation constant contact continuouswave contrast control convection convergence cooperative copolymer copper core core-shell corrections correlation coupled covalent cr crescent critical cro2 cross-section crystallization crystals ct ctab ctyoplasm cuo curie curve cvd cw cyanide cyclohexane cylindrical cytochrome cytometry cytoplasm dark-field data dbr dctp dda deactivation debye decanuclear decay deflection defocus deformable degradable delay delivery demagnetization denature dendrimers dendrites density depolarization depth design detection deuterium development devices dextrans dfb diagnosis diagnostics diamond dichroism dielectric dielectrophoretic diffraction diffuse diffusion dimers dimethylnaphthalene diode dioxide dipole directed directional discosoma discrete disk dispersion dispersions 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interface interference internal internet inverse invivo ion-beam ions ir iridium iron island isopropanol isotope isotropic j-aggregates janus kerr kinesin kinesin-5 kinetics knot kramers kronig label labonachip lab-on-a-chip laguerre-gaussian laminar large-field lasers latent lateral layers ld lead least-squares led left-handed length lens leukemia levitation lifetime lift light limit linear linewidth lipid liposome liquid liquid-crystal lithium lithography local-field localized locked locomotion lorentz lorenz-mie low-frequency low-pressure low-resolution low-reynolds low-solubility lspr luminescence magmoons magnesium magnetic magnetization magneto-optical magnetophoresis magnetostatic magnetotactic malaria maldi male manipulation markovian maser masks mason mass matched matlab matrix matter mbe mechanical medical medium mef membranes mems mercury mesoporous metallic metamaterials metaphase methylene mgo mice micelle microbes microchannel microchip microcrystals microdisks 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photoisomer photolabile photolithography photon photonics photoperoxidation photophysical photopolymerization photoresist photosensitizers photostability photo-switchable photosynthesis photothermolysis phototoxicity physical physics piconewton pi-conjugated picosecond piv planar plane plants plasma plasmon plasmons platelets pmma pn polarimeter polaritons polarizability polarization polyacrylamide polydispersity polyethylene polyethyleneglycol polyimides polylactide polymer polymerase polysilicon polystyrene ponderomotive population porous position positive postdoctoral posts potassium powder power precession presentations pressure printing prisms probability probes profile projection prolith2 properties prostate protein ptk2 pulses pump pump-probe pva pvp pyrene pyrromethene pyruvate qip quadropole quantum quantumdots quartz quench r6g rabi radial radiation radiationless radiative radius raman random rapid rat ratchets rate rattle rayleigh rayoptics reaction reactive rec-a receptors recognition red reflection reflectivity reflector refraction regime relaxation remote replication repricipitation repulsion research resist resistance resolution resonance resonator resonators response reversal reversible review reynolds rfp rheology rheometry rigid rigid-body ring r-matrix rna rod rolling room-temperature ros rotation rotational rotor roughness ruby rulers ruthenium sapphire saturation scan scattering science scientific screw search second section sedimentation selective self-assembly self-focused self-mode-locked semiconductor semiflexible sensing sensitivity sensitizers sensors sers serum shape shear shell shg shift shot-noise sialyl sic signals silica silicon silver silylation simulation single single-strand singlet singularity sio2 size skeleton slip slowly snom sodium software solar sol-gel solid-state solution solvent sorting sound spanners spatial spatiotemporal species specific spectrograph spectroscopy spectrum specular spheres spherical spheroidal spin 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transport trap tribology trilevel trimer triplet tsr tube tumor tunable tungsten tuning turmeric tweezers two-color two-photon ultrafast ultrasonics ultraviolet undergraduate unsaturated uofm upconversion u-tas uv uv-switchable vacuum vcsel vector velocimetry velocity vesicles viability vibrational video viruses viscoelasticity viscosity viscous visible vivo voltage volume vortex walk walls water watt wave waveguides wavelength waves web webb welding wgm whispering white-light wide-field width wigner wire wo3 wolfram wormlike wwii x-rays xuv xx yellow yield zeptogram zeroth zno




