A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions Export

@article{citeulike:1444510, abstract = {Specific, end-labeled DNA fragments can be simply and rapidly prepared using the polymerase chain reaction (PCR). Such fragments are suitable for use in DNase I protection footprint assays, chemical sequencing reactions, and for the production and analysis of paused RNA polymerase transcription complexes. Moreover, a general means of introducing a specific mutation at any position along the length of such PCR-generated fragments is described. These procedures, which can circumvent the need for large-scale phage or plasmid growths, preparative gel-electrophoresis and the screening of molecular clones, can facilitate the rapid study of sequence-specffic interactions of proteins and DNA. A rapid means of removing excess oligonucleotide primers from completed PCRs is also described. 10.1093/nar/16.15.7351}, author = {Higuchi, Russell and Krummel, Barbara and Saiki, Randall}, citeulike-article-id = {1444510}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/16.15.7351}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/content/16/15/7351.abstract}, citeulike-linkout-2 = {http://nar.oxfordjournals.org/content/16/15/7351.full.pdf}, citeulike-linkout-3 = {http://nar.oxfordjournals.org/cgi/content/abstract/16/15/7351}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/3045756}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=3045756}, day = {11}, doi = {10.1093/nar/16.15.7351}, journal = {Nucl. Acids Res.}, keywords = {directed, mutagenesis, pcr, site}, month = {August}, number = {15}, pages = {7351--7367}, posted-at = {2009-10-05 18:37:38}, priority = {2}, title = {A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions}, url = {http://dx.doi.org/10.1093/nar/16.15.7351}, volume = {16}, year = {1988} }

TY - JOUR ID - citeulike:1444510 L3 - citeulike-article-id:1444510 N2 - Specific, end-labeled DNA fragments can be simply and rapidly prepared using the polymerase chain reaction (PCR). Such fragments are suitable for use in DNase I protection footprint assays, chemical sequencing reactions, and for the production and analysis of paused RNA polymerase transcription complexes. Moreover, a general means of introducing a specific mutation at any position along the length of such PCR-generated fragments is described. These procedures, which can circumvent the need for large-scale phage or plasmid growths, preparative gel-electrophoresis and the screening of molecular clones, can facilitate the rapid study of sequence-specffic interactions of proteins and DNA. A rapid means of removing excess oligonucleotide primers from completed PCRs is also described. 10.1093/nar/16.15.7351 IS - 15 JF - Nucl. Acids Res. EP - 7367 TI - A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions VL - 16 SP - 7351 KW - directed KW - mutagenesis KW - pcr KW - site AU - Higuchi, Russell AU - Krummel, Barbara AU - Saiki, Randall PY - 1988/08/11/ UR - http://dx.doi.org/10.1093/nar/16.15.7351 ER -