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Mol Ecol, Vol. 22, No. 1. (1 January 2013), pp. 19-21, doi:10.1111/mec.12050 Key: citeulike:11867523
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It has been a tumultuous 5 years in phylogeography and phylogenetics during which both fields have struggled to harness the power of next-generation sequencing (NGS) (Ekblom & Galindo ; McCormack et al. ). Fortunately, several methodological approaches appear to be taking root. In this issue of Molecular Ecology, O'Neill et al. ) employ one such method – parallel tagged sequencing (PTS) – to elucidate the phylogeography of a tiger salamander (Ambystoma tigrinum) species complex. This study demonstrates a practical application of NGS on a scale appropriate (and not overkill) for most biologists interested in phylogeography (~100 loci for ~100 individuals), and their results highlight several analytical challenges that lie ahead for researchers employing NGS techniques.
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