Solvent Magnetization Artifacts in High-Field NMR Studies of Macromolecular Hydration Export

@article{citeulike:3372685, abstract = {With the use of high magnetic fields and improved quality factor ratings of the probeheads in modern NMR spectrometers, radiation damping becomes more and more important. In addition, the demagnetizing field effect from protonated solvents gains significance with the increase of the magnetic field strength. During a typical NMR pulse sequence the magnetic fields caused by these effects become time-dependent, which makes the system nonlinear and may, for example, measurably influence the precession frequencies of all nuclei in the sample. Since radiation damping can affect signals that are several kilohertz away from the solvent resonance, the amplitude, phase, and frequency of the desired signals can be disturbed so as to give rise to spectral artifacts. In particular when difference methods are used to obtain the final spectrum, the data sets may be severely deteriorated by such artifacts. This paper investigates effects from the demagnetizing field and from radiation damping with a selection of pulse sequences in use for studies of macromolecular hydration, and strategies are described for the detection and elimination of the ensuing artifacts.}, author = {Sobol, G. S. and Wider, G. and Iwai, H. and W\"{u}thrich, K.}, citeulike-article-id = {3372685}, journal = {Journal of Magnetic Resonance}, keywords = {bulk-magnetization, hydration, nmr-methods}, number = {2}, pages = {262--271}, posted-at = {2008-10-04 02:57:17}, priority = {2}, title = {Solvent Magnetization Artifacts in High-Field NMR Studies of Macromolecular Hydration}, volume = {130}, year = {1998} }

TY - JOUR ID - citeulike:3372685 L3 - citeulike-article-id:3372685 N2 - With the use of high magnetic fields and improved quality factor ratings of the probeheads in modern NMR spectrometers, radiation damping becomes more and more important. In addition, the demagnetizing field effect from protonated solvents gains significance with the increase of the magnetic field strength. During a typical NMR pulse sequence the magnetic fields caused by these effects become time-dependent, which makes the system nonlinear and may, for example, measurably influence the precession frequencies of all nuclei in the sample. Since radiation damping can affect signals that are several kilohertz away from the solvent resonance, the amplitude, phase, and frequency of the desired signals can be disturbed so as to give rise to spectral artifacts. In particular when difference methods are used to obtain the final spectrum, the data sets may be severely deteriorated by such artifacts. This paper investigates effects from the demagnetizing field and from radiation damping with a selection of pulse sequences in use for studies of macromolecular hydration, and strategies are described for the detection and elimination of the ensuing artifacts. IS - 2 JF - Journal of Magnetic Resonance EP - 271 TI - Solvent Magnetization Artifacts in High-Field NMR Studies of Macromolecular Hydration VL - 130 SP - 262 KW - bulk-magnetization KW - hydration KW - nmr-methods AU - Sobol, GS AU - Wider, G AU - Iwai, H AU - Wüthrich, K PY - 1998/// ER -