The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin. Export

@article{citeulike:3500676, abstract = {A number of large noncoding RNAs (ncRNAs) epigenetically silence genes through unknown mechanisms. The Air ncRNA is imprinted--monoallelically expressed from the paternal allele. Air is required for allele-specific silencing of the cis-linked Slc22a3, Slc22a2, and Igf2r genes in mouse placenta. We show that Air interacts with the Slc22a3 promoter chromatin and the H3K9 histone methyltransferase G9a in placenta. Air accumulates at the Slc22a3 promoter in correlation with localized H3K9 methylation and transcriptional repression. Genetic ablation of G9a results in nonimprinted, biallelic transcription of Slc22a3. Truncated Air fails to accumulate at the Slc22a3 promoter, which results in reduced G9a recruitment and biallelic transcription. Our results suggest that Air, and potentially other large ncRNAs, target repressive histone-modifying activities through molecular interaction with specific chromatin domains to epigenetically silence transcription.}, author = {Nagano, Takashi and Mitchell, Jennifer A. and Sanz, Lionel A. and Pauler, Florian M. and Ferguson-Smith, Anne C. and Feil, Robert and Fraser, Peter}, citeulike-article-id = {3500676}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1163802}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/1163802//}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18988810}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18988810}, day = {12}, doi = {10.1126/science.1163802}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {air, epigenetics, g9a, imprinting, rna, transcription}, month = {December}, number = {5908}, pages = {1717--1720}, posted-at = {2008-11-10 09:53:02}, priority = {2}, title = {The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin.}, url = {http://dx.doi.org/10.1126/science.1163802}, volume = {322}, year = {2008} }

TY - JOUR ID - citeulike:3500676 L3 - citeulike-article-id:3500676 N2 - A number of large noncoding RNAs (ncRNAs) epigenetically silence genes through unknown mechanisms. The Air ncRNA is imprinted--monoallelically expressed from the paternal allele. Air is required for allele-specific silencing of the cis-linked Slc22a3, Slc22a2, and Igf2r genes in mouse placenta. We show that Air interacts with the Slc22a3 promoter chromatin and the H3K9 histone methyltransferase G9a in placenta. Air accumulates at the Slc22a3 promoter in correlation with localized H3K9 methylation and transcriptional repression. Genetic ablation of G9a results in nonimprinted, biallelic transcription of Slc22a3. Truncated Air fails to accumulate at the Slc22a3 promoter, which results in reduced G9a recruitment and biallelic transcription. Our results suggest that Air, and potentially other large ncRNAs, target repressive histone-modifying activities through molecular interaction with specific chromatin domains to epigenetically silence transcription. IS - 5908 JF - Science (New York, N.Y.) SN - 1095-9203 EP - 1720 TI - The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin. VL - 322 SP - 1717 KW - air KW - epigenetics KW - g9a KW - imprinting KW - rna KW - transcription AU - Nagano, Takashi AU - Mitchell, Jennifer AU - Sanz, Lionel AU - Pauler, Florian AU - Ferguson-Smith, Anne AU - Feil, Robert AU - Fraser, Peter PY - 2008/12/12/ UR - http://dx.doi.org/10.1126/science.1163802 ER -