Stationary and flying single hot-wire measurements were made to investigate the near to intermediate field in a round free turbulent jet. Measurements were carried out at Reynolds numbers ReD based on the jet exit mean velocity and the nozzle diameter, between 6000 and 100 000. The objective of this study was to investigate the concept of a mixing transition proposed by Dimotakis [“The mixing transition in turbulent flows,” J. Fluid Mech. 409, 69 (2000)]. This was done through the measurement of the velocity spectra and the calculation of Kolmogorov K, Taylor microscale T, the Liepmann–Taylor microscale L, and the viscous length scale at different positions in the near to intermediate regions of a round free jet. The present results demonstrate the local nature of mixing transition. It is found that the Kolmogorov, Taylor, and viscous length scales all decrease in magnitude with the local Reynolds number Re based on the local center line mean velocity and the local time-averaged diameter of the jet and appear to be only weakly dependent on the radial position, although the ratio ReT/Re1/2 varies nonlinearly across the jet radius. The ratio of the laminar to viscous length scale exceeds unity beyond the potential core of the jet. Moreover, the skewness of the axial velocity is approximately −0.4 over 30<ReT<400.