QPRIMER: A Quick Web-based Application for Designing Conserved PCR Primers from Multigenome Alignments. Export

@article{Kim2007a, abstract = {SUMMARY: We have developed a quick web-based application for designing conserved genomic PCR and RT-PCR primers from multigenome alignments targeting specific exons or introns. We used Pygr (The Python Graph Database Framework for Bioinformatics) to query intervals from multigenome alignments, which gives us less than a millisecond access to any intervals of any genome within multigenome alignments. PRIMER3 was used to extract optimal primers from a gene of interest. QPRIMER creates an electronic genomic PCR image from a set of conserved primers as well as summary pages for primer alignments and products. QPRIMER supports human, mouse, rat, chicken, dog, zebrafish, and fruit fly. AVAILABILITY: http://www.bioinformatics.ucla.edu/QPRIMER/}, address = {Center for Computational Biology, Molecular Biology Institute, Institute for Genomics and Proteomics, Department of Chemistry and Biochemistry, University of California, Los Angeles, 90095-1570 USA.}, author = {Kim, Namshin and Lee, Christopher}, citeulike-article-id = {1444976}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/bioinformatics/btm343}, citeulike-linkout-1 = {http://bioinformatics.oxfordjournals.org/cgi/content/abstract/23/17/2331}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17599926}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17599926}, day = {28}, doi = {10.1093/bioinformatics/btm343}, issn = {1460-2059}, journal = {Bioinformatics}, keywords = {alignment, bio-tools, bioinformatics, primer-design, python}, month = {June}, posted-at = {2007-09-11 13:35:16}, priority = {2}, title = {QPRIMER: A Quick Web-based Application for Designing Conserved PCR Primers from Multigenome Alignments.}, url = {http://dx.doi.org/10.1093/bioinformatics/btm343}, year = {2007} }

TY - JOUR ID - Kim2007a L3 - citeulike-article-id:1444976 N2 - SUMMARY: We have developed a quick web-based application for designing conserved genomic PCR and RT-PCR primers from multigenome alignments targeting specific exons or introns. We used Pygr (The Python Graph Database Framework for Bioinformatics) to query intervals from multigenome alignments, which gives us less than a millisecond access to any intervals of any genome within multigenome alignments. PRIMER3 was used to extract optimal primers from a gene of interest. QPRIMER creates an electronic genomic PCR image from a set of conserved primers as well as summary pages for primer alignments and products. QPRIMER supports human, mouse, rat, chicken, dog, zebrafish, and fruit fly. AVAILABILITY: http://www.bioinformatics.ucla.edu/QPRIMER/ JF - Bioinformatics SN - 1460-2059 AD - Center for Computational Biology, Molecular Biology Institute, Institute for Genomics and Proteomics, Department of Chemistry and Biochemistry, University of California, Los Angeles, 90095-1570 USA. TI - QPRIMER: A Quick Web-based Application for Designing Conserved PCR Primers from Multigenome Alignments. KW - alignment KW - bio-tools KW - bioinformatics KW - primer-design KW - python AU - Kim, Namshin AU - Lee, Christopher PY - 2007/06/28/ UR - http://dx.doi.org/10.1093/bioinformatics/btm343 ER -