Simple biodosimetry method for cases of high-dose radiation exposure using the ratio of the longest/shortest length of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC) Export

@article{citeulike:274318, abstract = {The aim was to develop a simple biodosimetry method for as rapid as possible estimation of absorbed radiation doses in victims of radiation accidents, in particular after high-dose exposure. Human peripheral blood lymphocytes (PBL) were gamma-irradiated in vitro with several doses up to 40 Gy stimulated with phytohaemagglutinin-P (PHA-P) for 2 days and their chromosomes condensed prematurely using 50 nm calyculin A. Chromosome lengths of Giemsa-stained G2 prematurely condensed chromosomes (PCC) were measured using image analysing software and the ratio of the longest/shortest chromosome length was calculated. The length ratio (LR) of the longest/shortest Giemsa-stained chromosome s increased with a good correlation to the square root of the radiation dose ( D ) up to 40Gy, i.e. LR\&\#160;=\&\#160;(4.90 \&\#215; D 0.5 ) + 2.14. The LR of the longest/shortest chromosome might be used as an index for estimating the radiation dose. The blood samples should not be cooled until the start of separation/stimulation of the lymphocytes. A rapid and easy estimation of large doses after whole-body exposure was identified by measuring the ratio of the longest/shortest length of Giemsa-stained G2-PCC induced by calyculin A. This simple protocol will be particularly useful for making therapy decisions for victims of ionizing radiation exposure and has potential for use as a biodosimeter for partial-body exposure accidents.}, author = {Gotoh, E. and Tanno, Y.}, citeulike-article-id = {274318}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/09553000500147667}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/tandf/trab/2005/00000081/00000005/art00005}, doi = {10.1080/09553000500147667}, issn = {0955-3002}, journal = {International Journal of Radiation Biology}, keywords = {biodosimetry, pcc}, month = {May}, number = {5}, pages = {379--385}, posted-at = {2009-09-24 13:36:22}, priority = {2}, publisher = {Informa Healthcare}, title = {Simple biodosimetry method for cases of high-dose radiation exposure using the ratio of the longest/shortest length of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC)}, url = {http://dx.doi.org/10.1080/09553000500147667}, volume = {81}, year = {2005} }

TY - JOUR ID - citeulike:274318 L3 - citeulike-article-id:274318 N2 - The aim was to develop a simple biodosimetry method for as rapid as possible estimation of absorbed radiation doses in victims of radiation accidents, in particular after high-dose exposure. Human peripheral blood lymphocytes (PBL) were gamma-irradiated in vitro with several doses up to 40 Gy stimulated with phytohaemagglutinin-P (PHA-P) for 2 days and their chromosomes condensed prematurely using 50 nm calyculin A. Chromosome lengths of Giemsa-stained G2 prematurely condensed chromosomes (PCC) were measured using image analysing software and the ratio of the longest/shortest chromosome length was calculated. The length ratio (LR) of the longest/shortest Giemsa-stained chromosome s increased with a good correlation to the square root of the radiation dose ( D ) up to 40Gy, i.e. LR = (4.90 × D 0.5 ) + 2.14. The LR of the longest/shortest chromosome might be used as an index for estimating the radiation dose. The blood samples should not be cooled until the start of separation/stimulation of the lymphocytes. A rapid and easy estimation of large doses after whole-body exposure was identified by measuring the ratio of the longest/shortest length of Giemsa-stained G2-PCC induced by calyculin A. This simple protocol will be particularly useful for making therapy decisions for victims of ionizing radiation exposure and has potential for use as a biodosimeter for partial-body exposure accidents. IS - 5 JF - International Journal of Radiation Biology SN - 0955-3002 EP - 385 TI - Simple biodosimetry method for cases of high-dose radiation exposure using the ratio of the longest/shortest length of Giemsa-stained drug-induced prematurely condensed chromosomes (PCC) PB - Informa Healthcare VL - 81 SP - 379 KW - biodosimetry KW - pcc AU - Gotoh, E AU - Tanno, Y PY - 2005/05// UR - http://dx.doi.org/10.1080/09553000500147667 ER -