Chromosomal intrachanges induced by swift iron ions. Export

@article{citeulike:2931213, abstract = {We measured the induction of structural aberrations in human chromosome 5 induced by iron ions using the novel technique of multicolor banding in situ hybridization (mBAND). Human lymphocytes isolated from whole blood were exposed in vitro to 500 MeV/n (LET=200 keV/micrometers, doses 1 or 4 Gy) Fe nuclei at the HIMAC accelerator in Chiba (Japan). Chromosomes were prematurely condensed by calyculin A after 48 h in culture and slides were painted by mBAND. We found a frequency of 0.11 and 0.57 residual breakpoints per chromosome 5 after 1 and 4 Gy Fe-ions, respectively. Inter-chromosomal exchanges were the prevalent aberration type measured at both doses, followed by terminal deletions, and by intra-chromosomal exchanges. Among intra-chromosomal exchanges, intra-arm events were more frequent than inter-arm, but a significant number of intra-changes was associated to inter-changes involving the same chromosome after 4 Gy of iron ions. These events show that the complexity of chromosomal exchanges induced by heavy ions can be higher than expected by previous FISH studies.}, address = {Department of Genetics, University of Duisburg-Essen, Essen, Germany.}, author = {Hortsmann, M. and Durante, M. and Johannes, C. and Obe, G.}, citeulike-article-id = {2931213}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/15934206}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=15934206}, issn = {0273-1177}, journal = {Advances in space research : the official journal of the Committee on Space Research (COSPAR)}, keywords = {fe-ion, mband}, number = {2}, pages = {276--279}, posted-at = {2008-06-26 15:43:12}, priority = {2}, title = {Chromosomal intrachanges induced by swift iron ions.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15934206}, volume = {35}, year = {2005} }

TY - JOUR ID - citeulike:2931213 L3 - citeulike-article-id:2931213 N2 - We measured the induction of structural aberrations in human chromosome 5 induced by iron ions using the novel technique of multicolor banding in situ hybridization (mBAND). Human lymphocytes isolated from whole blood were exposed in vitro to 500 MeV/n (LET=200 keV/micrometers, doses 1 or 4 Gy) Fe nuclei at the HIMAC accelerator in Chiba (Japan). Chromosomes were prematurely condensed by calyculin A after 48 h in culture and slides were painted by mBAND. We found a frequency of 0.11 and 0.57 residual breakpoints per chromosome 5 after 1 and 4 Gy Fe-ions, respectively. Inter-chromosomal exchanges were the prevalent aberration type measured at both doses, followed by terminal deletions, and by intra-chromosomal exchanges. Among intra-chromosomal exchanges, intra-arm events were more frequent than inter-arm, but a significant number of intra-changes was associated to inter-changes involving the same chromosome after 4 Gy of iron ions. These events show that the complexity of chromosomal exchanges induced by heavy ions can be higher than expected by previous FISH studies. IS - 2 JF - Advances in space research : the official journal of the Committee on Space Research (COSPAR) SN - 0273-1177 AD - Department of Genetics, University of Duisburg-Essen, Essen, Germany. EP - 279 TI - Chromosomal intrachanges induced by swift iron ions. VL - 35 SP - 276 KW - fe-ion KW - mband AU - Hortsmann, M AU - Durante, M AU - Johannes, C AU - Obe, G PY - 2005/// UR - http://view.ncbi.nlm.nih.gov/pubmed/15934206 ER -