Activation of NF-κB in bone marrow cells of BALB/cJ mice following exposure in vivo to low doses of 137Cs γ-rays Export

@article{citeulike:3420366, abstract = {Abstract\ \ We measured levels of NF-κB activation in bone marrow (BM) cells collected at 1 and 4\ h from male BALB/cJ mice (10–12\ weeks old) given a whole body dose of 0, 0.05, 0.1 and 1\ Gy of 137Cs γ-rays (at the dose rate of 0.75\ Gy/min). At each harvest time-point, BM cells were collected from five mice per dose of radiation. We used two methods for detecting NF-κB activation (1) the NF-κB/p65 transcription factor enzyme-linked immunosorbance assay (ELISA) and (2) immunofluorescence staining with NF-κB/p65 antibody. Results from ELISA indicated 2.0 and 2.8-fold increases in NF-κB activation in BM cells isolated at 1\ h post-exposure of mice to 0.1 or 1.0\ Gy. The immunofluorescence staining method showed similar results. In samples isolated 4\ h post-irradiation, however, no activated NF-κB signal was found, regardless of the method of detection. The data also demonstrated that NF-κB was not activated in bone marrow cells collected either at 1 or 4\ h from BALB/cJ mice exposed to a single dose of 0.05\ Gy 137Cs γ-rays. Taken together, the results from our in vivo study indicate the involvement of NF-κB activation in early response to 0.1 and 1.0\ Gy (but not 0.05\ Gy) of 137Cs γ-rays.}, author = {Rithidech, Kanokporn and Tungjai, Montree and Arbab, Edgar and Simon, Sanford}, citeulike-article-id = {3420366}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00411-005-0004-5}, day = {1}, doi = {10.1007/s00411-005-0004-5}, journal = {Radiation and Environmental Biophysics}, keywords = {mice}, month = {October}, number = {2}, pages = {139--143}, posted-at = {2008-10-16 13:52:42}, priority = {2}, title = {Activation of NF-κB in bone marrow cells of BALB/cJ mice following exposure in vivo to low doses of 137Cs γ-rays}, url = {http://dx.doi.org/10.1007/s00411-005-0004-5}, volume = {44}, year = {2005} }

TY - JOUR ID - citeulike:3420366 L3 - citeulike-article-id:3420366 N2 - Abstract  We measured levels of NF-κB activation in bone marrow (BM) cells collected at 1 and 4 h from male BALB/cJ mice (10–12 weeks old) given a whole body dose of 0, 0.05, 0.1 and 1 Gy of 137Cs γ-rays (at the dose rate of 0.75 Gy/min). At each harvest time-point, BM cells were collected from five mice per dose of radiation. We used two methods for detecting NF-κB activation (1) the NF-κB/p65 transcription factor enzyme-linked immunosorbance assay (ELISA) and (2) immunofluorescence staining with NF-κB/p65 antibody. Results from ELISA indicated 2.0 and 2.8-fold increases in NF-κB activation in BM cells isolated at 1 h post-exposure of mice to 0.1 or 1.0 Gy. The immunofluorescence staining method showed similar results. In samples isolated 4 h post-irradiation, however, no activated NF-κB signal was found, regardless of the method of detection. The data also demonstrated that NF-κB was not activated in bone marrow cells collected either at 1 or 4 h from BALB/cJ mice exposed to a single dose of 0.05 Gy 137Cs γ-rays. Taken together, the results from our in vivo study indicate the involvement of NF-κB activation in early response to 0.1 and 1.0 Gy (but not 0.05 Gy) of 137Cs γ-rays. IS - 2 JF - Radiation and Environmental Biophysics EP - 143 TI - Activation of NF-κB in bone marrow cells of BALB/cJ mice following exposure in vivo to low doses of 137Cs γ-rays VL - 44 SP - 139 KW - mice AU - Rithidech, Kanokporn AU - Tungjai, Montree AU - Arbab, Edgar AU - Simon, Sanford PY - 2005/10/01/ UR - http://dx.doi.org/10.1007/s00411-005-0004-5 ER -