Karyotypes of human lymphocytes exposed to high-energy iron ions. Export

@article{citeulike:5298867, abstract = {Chromosomal aberrations were analyzed using multicolor fluorescence in situ hybridization (mFISH) in human peripheral blood lymphocytes after in vitro exposure to gamma rays or accelerated (56)Fe ions (1 GeV/nucleon, 145 keV/microm) at Brookhaven National Laboratory (Upton, NY). Doses of 0.3 and 3 Gy were used for both radiation types. Chromosomes were prematurely condensed by a phosphatase inhibitor (calyculin A) to avoid the population selection bias observed at metaphase as a result of the severe cell cycle delays induced by heavy ions. A total of 1053 karyotypes (G(2) and M phases) were analyzed in irradiated lymphocytes. Results revealed different distribution patterns for chromosomal aberrations after low- and high-LET radiation exposures: Heavy ions induced a much higher fraction of cells with multiple aberrations, while the majority of the aberrant cells induced by low doses of gamma rays contained a single aberration. The high fraction of complex-type exchanges after heavy ions leads to an overestimation of simple-type asymmetrical interchanges (dicentrics) from analysis of Giemsa-stained samples. However, even after a dose of 3 Gy iron ions, about 30\% of the cells presented no complex-type exchanges. The involvement of individual chromosomes in exchanges was similar for densely and sparsely ionizing radiation, and no statistically significant evidence of a nonrandom involvement of specific chromosomes was detected.}, author = {Durante, M. and George, K. and Wu, H. and Cucinotta, F. A.}, citeulike-article-id = {5298867}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12385635}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12385635}, issn = {0033-7587}, journal = {Radiation research}, keywords = {fe-ion, mfish}, month = {November}, number = {5}, pages = {581--590}, posted-at = {2009-07-29 15:27:36}, priority = {2}, title = {Karyotypes of human lymphocytes exposed to high-energy iron ions.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12385635}, volume = {158}, year = {2002} }

TY - JOUR ID - citeulike:5298867 L3 - citeulike-article-id:5298867 N2 - Chromosomal aberrations were analyzed using multicolor fluorescence in situ hybridization (mFISH) in human peripheral blood lymphocytes after in vitro exposure to gamma rays or accelerated (56)Fe ions (1 GeV/nucleon, 145 keV/microm) at Brookhaven National Laboratory (Upton, NY). Doses of 0.3 and 3 Gy were used for both radiation types. Chromosomes were prematurely condensed by a phosphatase inhibitor (calyculin A) to avoid the population selection bias observed at metaphase as a result of the severe cell cycle delays induced by heavy ions. A total of 1053 karyotypes (G(2) and M phases) were analyzed in irradiated lymphocytes. Results revealed different distribution patterns for chromosomal aberrations after low- and high-LET radiation exposures: Heavy ions induced a much higher fraction of cells with multiple aberrations, while the majority of the aberrant cells induced by low doses of gamma rays contained a single aberration. The high fraction of complex-type exchanges after heavy ions leads to an overestimation of simple-type asymmetrical interchanges (dicentrics) from analysis of Giemsa-stained samples. However, even after a dose of 3 Gy iron ions, about 30% of the cells presented no complex-type exchanges. The involvement of individual chromosomes in exchanges was similar for densely and sparsely ionizing radiation, and no statistically significant evidence of a nonrandom involvement of specific chromosomes was detected. IS - 5 JF - Radiation research SN - 0033-7587 EP - 590 TI - Karyotypes of human lymphocytes exposed to high-energy iron ions. VL - 158 SP - 581 KW - fe-ion KW - mfish AU - Durante, M AU - George, K AU - Wu, H AU - Cucinotta, FA PY - 2002/11// UR - http://view.ncbi.nlm.nih.gov/pubmed/12385635 ER -