Identification of X-ray-induced complex chromosome exchanges using fluorescence in situ hybridization: a comparison at two doses. Export

@article{citeulike:5906673, abstract = {Complex chromosome exchanges are defined as interactions between three or more breaks, in two or more chromosomes. In this study, a sequential hybridisation technique was developed to visualize a given chromosome pair: green (chromosomes 1, 5 and 7), all centromeres red and the remaining chromosomes blue. Primary human fibroblasts were irradiated in G1 with 4 and 6 Gy 250-kV X-rays. After 4 Gy, a total of 387 simple aberrations (defined as translocations or dicentrics with fragments) and 116 complex aberrations were identified. After 6 Gy these aberrations numbered 225 and 110 respectively. Using a break-rejoin scheme, which describes interactions between breaks within a complex as independent events we modelled the complex 'mix' present after 4 Gy. At 6 Gy the same model could be used for chromosomes 5 and 7, but chromosome 1 frequencies could only be explained if we biased the interactions, such that two-breaks-in-one-chromosome events occur predominantly in chromosome 1. From these predicted interactions we also calculated the number of apparently simple exchanges that are actually complex derived. These accounted for 20\% of those observed after 4 Gy and 33\% after 6 Gy. Therefore, with this FISH assay, an estimated 35 and 54\% of all exchanges are derived from complexes after 4 and 6 Gy respectively.}, author = {Simpson, P. J. and Savage, J. R.}, citeulike-article-id = {5906673}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7983457}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7983457}, issn = {0955-3002}, journal = {International journal of radiation biology}, keywords = {complex, fish, x-rays}, month = {November}, number = {5}, pages = {629--632}, posted-at = {2009-10-07 17:44:55}, priority = {2}, title = {Identification of X-ray-induced complex chromosome exchanges using fluorescence in situ hybridization: a comparison at two doses.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7983457}, volume = {66}, year = {1994} }

TY - JOUR ID - citeulike:5906673 L3 - citeulike-article-id:5906673 N2 - Complex chromosome exchanges are defined as interactions between three or more breaks, in two or more chromosomes. In this study, a sequential hybridisation technique was developed to visualize a given chromosome pair: green (chromosomes 1, 5 and 7), all centromeres red and the remaining chromosomes blue. Primary human fibroblasts were irradiated in G1 with 4 and 6 Gy 250-kV X-rays. After 4 Gy, a total of 387 simple aberrations (defined as translocations or dicentrics with fragments) and 116 complex aberrations were identified. After 6 Gy these aberrations numbered 225 and 110 respectively. Using a break-rejoin scheme, which describes interactions between breaks within a complex as independent events we modelled the complex 'mix' present after 4 Gy. At 6 Gy the same model could be used for chromosomes 5 and 7, but chromosome 1 frequencies could only be explained if we biased the interactions, such that two-breaks-in-one-chromosome events occur predominantly in chromosome 1. From these predicted interactions we also calculated the number of apparently simple exchanges that are actually complex derived. These accounted for 20% of those observed after 4 Gy and 33% after 6 Gy. Therefore, with this FISH assay, an estimated 35 and 54% of all exchanges are derived from complexes after 4 and 6 Gy respectively. IS - 5 JF - International journal of radiation biology SN - 0955-3002 EP - 632 TI - Identification of X-ray-induced complex chromosome exchanges using fluorescence in situ hybridization: a comparison at two doses. VL - 66 SP - 629 KW - complex KW - fish KW - x-rays AU - Simpson, PJ AU - Savage, JR PY - 1994/11// UR - http://view.ncbi.nlm.nih.gov/pubmed/7983457 ER -