Easy biodosimetry for high-dose radiation exposures using drug-induced, prematurely condensed chromosomes. Export

@article{citeulike:6067065, abstract = {PURPOSE: To propose a practical method of biodosimetry for high-dose estimation. MATERIALS AND METHODS: Okadaic acid in the range 100 nM to 500 nM was given for 1-6 h to cultured lymphocytes that had been exposed in the range of 0 Gy to 40 Gy. The frequency of the cells with prematurely condensed chromosomes (PCC) was compared with that of metaphases accumulated by 0.05 microg/ml colcemid. The dose-response relationship of the yield of prematurely condensed ring chromosomes (PCC rings) in the irradiated lymphocytes was examined. RESULTS: The 0.05 microg/ml colcemid collected few metaphases when lymphocytes were irradiated to over 10 Gy. However, 500 nM okadaic acid produced a substantial number of analysable PCC in lymphocytes that had been exposed in the range of 0 Gy to 40 Gy. After 1 h of treatment, the 500 nM okadaic acid gave the most suitable chromosome morphology for the analysis. PCC rings were narrow, which facilitated their detection in Giemsa-stained preparations. The yield of PCC rings increased dose-dependently up to 20 Gy. CONCLUSION: PCC rings are a more suitable indicator than dicentrics at high doses over 10 Gy. This technique requires no particular equipment or exceptional skill. After a serious radiation accident, it can be easily performed in a short time in most medical facilities to obtain dose information to assist physicians.}, author = {Kanda, R. and Hayata, I. and Lloyd, D. C.}, citeulike-article-id = {6067065}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10331849}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10331849}, issn = {0955-3002}, journal = {International journal of radiation biology}, keywords = {biodosimetry, pcc}, month = {April}, number = {4}, pages = {441--446}, posted-at = {2009-11-04 09:08:08}, priority = {2}, title = {Easy biodosimetry for high-dose radiation exposures using drug-induced, prematurely condensed chromosomes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10331849}, volume = {75}, year = {1999} }

TY - JOUR ID - citeulike:6067065 L3 - citeulike-article-id:6067065 N2 - PURPOSE: To propose a practical method of biodosimetry for high-dose estimation. MATERIALS AND METHODS: Okadaic acid in the range 100 nM to 500 nM was given for 1-6 h to cultured lymphocytes that had been exposed in the range of 0 Gy to 40 Gy. The frequency of the cells with prematurely condensed chromosomes (PCC) was compared with that of metaphases accumulated by 0.05 microg/ml colcemid. The dose-response relationship of the yield of prematurely condensed ring chromosomes (PCC rings) in the irradiated lymphocytes was examined. RESULTS: The 0.05 microg/ml colcemid collected few metaphases when lymphocytes were irradiated to over 10 Gy. However, 500 nM okadaic acid produced a substantial number of analysable PCC in lymphocytes that had been exposed in the range of 0 Gy to 40 Gy. After 1 h of treatment, the 500 nM okadaic acid gave the most suitable chromosome morphology for the analysis. PCC rings were narrow, which facilitated their detection in Giemsa-stained preparations. The yield of PCC rings increased dose-dependently up to 20 Gy. CONCLUSION: PCC rings are a more suitable indicator than dicentrics at high doses over 10 Gy. This technique requires no particular equipment or exceptional skill. After a serious radiation accident, it can be easily performed in a short time in most medical facilities to obtain dose information to assist physicians. IS - 4 JF - International journal of radiation biology SN - 0955-3002 EP - 446 TI - Easy biodosimetry for high-dose radiation exposures using drug-induced, prematurely condensed chromosomes. VL - 75 SP - 441 KW - biodosimetry KW - pcc AU - Kanda, R AU - Hayata, I AU - Lloyd, DC PY - 1999/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/10331849 ER -