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In Journal of Biomolecular NMR, Vol. 2, No. 2. (1992), pp. 195-202, doi:10.1007/bf01875530 Key: citeulike:12076458
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A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C,15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.
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