THE GREEN FLUORESCENT PROTEIN Export

@article{citeulike:1060017, abstract = {▪ Abstract  In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.}, address = {Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093-0647, USA.}, author = {Tsien, Roger Y.}, citeulike-article-id = {1060017}, citeulike-linkout-0 = {http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.biochem.67.1.509}, citeulike-linkout-1 = {http://dx.doi.org/10.1146/annurev.biochem.67.1.509}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/9759496}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=9759496}, doi = {10.1146/annurev.biochem.67.1.509}, issn = {0066-4154}, journal = {Annual Review of Biochemistry}, keywords = {gfp, review}, number = {1}, pages = {509--544}, posted-at = {2009-06-08 21:36:29}, priority = {2}, title = {THE GREEN FLUORESCENT PROTEIN}, url = {http://dx.doi.org/10.1146/annurev.biochem.67.1.509}, volume = {67}, year = {1998} }

TY - JOUR ID - citeulike:1060017 L3 - citeulike-article-id:1060017 N2 - ▪ Abstract  In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories. IS - 1 JF - Annual Review of Biochemistry SN - 0066-4154 AD - Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093-0647, USA. EP - 544 TI - THE GREEN FLUORESCENT PROTEIN VL - 67 SP - 509 KW - gfp KW - review AU - Tsien, Roger PY - 1998/// UR - http://dx.doi.org/10.1146/annurev.biochem.67.1.509 ER -