Single-Molecule Fluorescence Resonance Energy Transfer Export

@article{citeulike:1146027, abstract = {Fluorescent resonance energy transfer (FRET) is a powerful technique for studying conformational distribution and dynamics of biological molecules. Some conformational changes are difficult to synchronize or too rare to detect using ensemble FRET. FRET, detected at the single-molecule level, opens up new opportunities to probe the detailed kinetics of structural changes without the need for synchronization. Here, we discuss practical considerations for its implementation including experimental apparatus, fluorescent probe selection, surface immobilization, single-molecule FRET analysis schemes, and interpretation. }, address = {Department of Physics, Center for Biophysics and Computational Biology, Urbana 61801, USA. tjha@uiuc.edu}, author = {Ha, T.}, citeulike-article-id = {1146027}, citeulike-linkout-0 = {http://dx.doi.org/10.1006/meth.2001.1217}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1046-2023(01)91217-4}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11558999}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11558999}, citeulike-linkout-4 = {http://www.sciencedirect.com/science/article/B6WN5-456JRGD-M/2/10e54250096b63b2bdee366f4fcdc1fd}, doi = {10.1006/meth.2001.1217}, issn = {10462023}, journal = {Methods}, keywords = {fret, review, sms}, month = {September}, number = {1}, pages = {78--86}, posted-at = {2009-11-04 19:10:12}, priority = {2}, title = {Single-Molecule Fluorescence Resonance Energy Transfer}, url = {http://dx.doi.org/10.1006/meth.2001.1217}, volume = {25}, year = {2001} }

TY - JOUR ID - citeulike:1146027 L3 - citeulike-article-id:1146027 N2 - Fluorescent resonance energy transfer (FRET) is a powerful technique for studying conformational distribution and dynamics of biological molecules. Some conformational changes are difficult to synchronize or too rare to detect using ensemble FRET. FRET, detected at the single-molecule level, opens up new opportunities to probe the detailed kinetics of structural changes without the need for synchronization. Here, we discuss practical considerations for its implementation including experimental apparatus, fluorescent probe selection, surface immobilization, single-molecule FRET analysis schemes, and interpretation. IS - 1 JF - Methods SN - 10462023 AD - Department of Physics, Center for Biophysics and Computational Biology, Urbana 61801, USA. tjha@uiuc.edu EP - 86 TI - Single-Molecule Fluorescence Resonance Energy Transfer VL - 25 SP - 78 KW - fret KW - review KW - sms AU - Ha, T PY - 2001/09// UR - http://dx.doi.org/10.1006/meth.2001.1217 ER -