Oxygen-dependent photochemistry of fluorescent dyes studied at the single molecule level Export

@article{citeulike:2387464, abstract = {We perform wide-field microscopy to investigate the photobleaching of organic fluorophores embedded in the polymeric host PMMA. Our experimental arrangement facilitates the comparison between the ensemble and single molecule data. We characterize the photostability of dye molecules of various families by measuring the \‘bleaching number\’, defined as the average number of photons a molecule emits until photobleaching occurs. In particular, we have analysed the dependence of the bleaching number on the presence of oxygen. Surprisingly, we find an improvement of photostability in the presence of oxygen for ionic dyes (DiI, TMR, Rh6G, Alexa 546), suggesting that oxygen quenches the photoactive triplet state, but it only indirectly contributes to photochemistry. In contrast, we observe that photobleaching of the aromatic hydrocarbon is strongly enhanced by oxygen.}, author = {Renn, Alois and Seelig, Johannes and Sandoghdar, Vahid}, citeulike-article-id = {2387464}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/00268970500361861}, doi = {10.1080/00268970500361861}, journal = {Molecular Physics}, keywords = {oxygen-scavenging, photobleaching, sms}, number = {3}, pages = {409--414}, posted-at = {2008-02-16 02:10:13}, priority = {0}, publisher = {Taylor \& Francis}, title = {Oxygen-dependent photochemistry of fluorescent dyes studied at the single molecule level}, url = {http://dx.doi.org/10.1080/00268970500361861}, volume = {104}, year = {2006} }

TY - JOUR ID - citeulike:2387464 L3 - citeulike-article-id:2387464 N2 - We perform wide-field microscopy to investigate the photobleaching of organic fluorophores embedded in the polymeric host PMMA. Our experimental arrangement facilitates the comparison between the ensemble and single molecule data. We characterize the photostability of dye molecules of various families by measuring the ‘bleaching number’, defined as the average number of photons a molecule emits until photobleaching occurs. In particular, we have analysed the dependence of the bleaching number on the presence of oxygen. Surprisingly, we find an improvement of photostability in the presence of oxygen for ionic dyes (DiI, TMR, Rh6G, Alexa 546), suggesting that oxygen quenches the photoactive triplet state, but it only indirectly contributes to photochemistry. In contrast, we observe that photobleaching of the aromatic hydrocarbon is strongly enhanced by oxygen. IS - 3 JF - Molecular Physics EP - 414 TI - Oxygen-dependent photochemistry of fluorescent dyes studied at the single molecule level PB - Taylor & Francis VL - 104 SP - 409 KW - oxygen-scavenging KW - photobleaching KW - sms AU - Renn, Alois AU - Seelig, Johannes AU - Sandoghdar, Vahid PY - 2006/// UR - http://dx.doi.org/10.1080/00268970500361861 ER -