The importance of surfaces in single-molecule bioscience

by: Mari L Visnapuu, Daniel Duzdevich, Eric C Greene

Molecular BioSystems, Vol. 4, No. 5. (2008), pp. 394-403.

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Abstract

The last ten years have witnessed an explosion of new techniques that can be used to probe the dynamic behavior of individual biological molecules, leading to discoveries that would not have been possible with more traditional biochemical methods. A common feature among these single-molecule approaches is the need for the biological molecules to be anchored to a solid support surface. This must be done under conditions that minimize nonspecific adsorption without compromising the biological integrity of the sample. In this review we highlight why surface attachments are a critical aspect of many single-molecule studies and we discuss current methods for anchoring biomolecules. Finally, we provide a detailed description of a new method developed by our laboratory for anchoring and organizing hundreds of individual DNA molecules on a surface, allowing 'high-throughput' studies of protein-DNA interactions at the single-molecule level.

@article{"b800444g", abstract = {The last ten years have witnessed an explosion of new techniques that can be used to probe the dynamic behavior of individual biological molecules, leading to discoveries that would not have been possible with more traditional biochemical methods. A common feature among these single-molecule approaches is the need for the biological molecules to be anchored to a solid support surface. This must be done under conditions that minimize nonspecific adsorption without compromising the biological integrity of the sample. In this review we highlight why surface attachments are a critical aspect of many single-molecule studies and we discuss current methods for anchoring biomolecules. Finally, we provide a detailed description of a new method developed by our laboratory for anchoring and organizing hundreds of individual DNA molecules on a surface, allowing 'high-throughput' studies of protein-DNA interactions at the single-molecule level.}, author = {Visnapuu, Mari L. and Duzdevich, Daniel and Greene, Eric C. }, citeulike-article-id = {2747101}, doi = {10.1039/b800444g}, journal = {Molecular BioSystems}, keywords = {review, sms}, number = {5}, pages = {394--403}, posted-at = {2008-05-02 22:24:43}, priority = {2}, title = {The importance of surfaces in single-molecule bioscience}, url = {http://dx.doi.org/10.1039/b800444g}, volume = {4}, year = {2008} }

RIS record

TY - JOUR ID - "b800444g" L3 - citeulike-article-id:2747101 TI - The importance of surfaces in single-molecule bioscience JF - Molecular BioSystems VL - 4 IS - 5 SP - 394 EP - 403 N2 - The last ten years have witnessed an explosion of new techniques that can be used to probe the dynamic behavior of individual biological molecules, leading to discoveries that would not have been possible with more traditional biochemical methods. A common feature among these single-molecule approaches is the need for the biological molecules to be anchored to a solid support surface. This must be done under conditions that minimize nonspecific adsorption without compromising the biological integrity of the sample. In this review we highlight why surface attachments are a critical aspect of many single-molecule studies and we discuss current methods for anchoring biomolecules. Finally, we provide a detailed description of a new method developed by our laboratory for anchoring and organizing hundreds of individual DNA molecules on a surface, allowing 'high-throughput' studies of protein-DNA interactions at the single-molecule level. KW - review KW - sms AU - Visnapuu, Mari AU - Duzdevich, Daniel AU - Greene, Eric PY - 2008/// UR - http://dx.doi.org/10.1039/b800444g ER -

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