Fluorescence Imaging of Membrane Dynamics Export

@article{citeulike:3035920, abstract = {Imaging membrane dynamics is an important goal, motivated by the abundance of biochemical and biophysical events that are orchestrated at, or by, cellular membranes. The short length scales, fast timescales, and environmental requirements of membrane phenomena present challenges to imaging experiments. Several technical advances offer means to overcome these challenges, and we describe here three powerful techniques applicable to membrane imaging: total internal reflection fluorescence (TIRF) microscopy, fluorescence interference contrast (FLIC) microscopy, and fluorescence correlation spectroscopy (FCS). For each, we discuss the physics underpinning the approach, its practical implementation, and recent examples highlighting its achievements in exploring the membrane environment.}, author = {Groves, Jay T. and Parthasarathy, Raghuveer and Forstner, Martin B.}, citeulike-article-id = {3035920}, citeulike-linkout-0 = {http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.bioeng.10.061807.160431}, citeulike-linkout-1 = {http://dx.doi.org/10.1146/annurev.bioeng.10.061807.160431}, doi = {10.1146/annurev.bioeng.10.061807.160431}, issn = {1545-4274}, journal = {Annual Review of Biomedical Engineering}, keywords = {membrane, microscopy, review}, month = {August}, number = {1}, pages = {311--338}, posted-at = {2009-06-09 23:49:37}, priority = {2}, title = {Fluorescence Imaging of Membrane Dynamics}, url = {http://dx.doi.org/10.1146/annurev.bioeng.10.061807.160431}, volume = {10}, year = {2008} }

TY - JOUR ID - citeulike:3035920 L3 - citeulike-article-id:3035920 N2 - Imaging membrane dynamics is an important goal, motivated by the abundance of biochemical and biophysical events that are orchestrated at, or by, cellular membranes. The short length scales, fast timescales, and environmental requirements of membrane phenomena present challenges to imaging experiments. Several technical advances offer means to overcome these challenges, and we describe here three powerful techniques applicable to membrane imaging: total internal reflection fluorescence (TIRF) microscopy, fluorescence interference contrast (FLIC) microscopy, and fluorescence correlation spectroscopy (FCS). For each, we discuss the physics underpinning the approach, its practical implementation, and recent examples highlighting its achievements in exploring the membrane environment. IS - 1 JF - Annual Review of Biomedical Engineering SN - 1545-4274 EP - 338 TI - Fluorescence Imaging of Membrane Dynamics VL - 10 SP - 311 KW - membrane KW - microscopy KW - review AU - Groves, Jay AU - Parthasarathy, Raghuveer AU - Forstner, Martin PY - 2008/08// UR - http://dx.doi.org/10.1146/annurev.bioeng.10.061807.160431 ER -